The JAX Cre Repository's aim is to provide the scientific community with a centralized, comprehensive set of well-characterized Cre Driver lines and related information resources. Available strains include Cre expressing strains, inducible Cre strains, Cre reporter strains, and loxP-flanked (floxed) strains.
The Cre Repository is supported by the Office of Research Infrastructure Programs/Office of the Director grant number OD011190 of the National Institutes of Health (NIH). Please reference this grant in citations acknowledging use of the resource.
The JAX Cre Repository uses a lacZ Cre-reporter strain, B6.129S4-Gt(ROSA)26Sortm1Sor/J, to assess cre activity. β-Galactosidase staining of 10.5 dpc embryos is done in whole mount. Because of tissue size, 15.5 dpc embryos, postnatal day 7 pups, and 8 week old adult mice are cryo-sectioned prior to staining of frozen sections.
The Jackson Laboratory offers over 300 cre tool strains. Listed by promoter, including site of expression:
Cre Reporter strains allow the user to assess the function (degree of excision) of an individual Cre line. These strains are engineered to express a reporter gene (such as lacZ or GFP) following the removal of a loxP-flanked STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. Some lines also express a distinct reporter prior to Cre-mediated excision. The JAX repository provides a wide selection of cre reporter lines that incorporate distinct reporter molecules.
Murray SA, Eppig JT, Smedley D, Simpson EM, Rosenthal N. 2012. Beyond knockouts: cre resources for conditional mutagenesis. Mamm Genome 23(9-10):587-599. PMCID:PMC3655717
Heffner CS, Pratt CH, Babiuk RP, Sharma Y, Rockwood SF, Donahue LR, Eppig JT, Murray SA. 2012. Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource. Nat Commun 3(1218):doi:10.1038/ncomms2186. PMCID: PMC3514490