With JAX, you can be confident your new model is precise and the genetic modification has been verified. We use a variety of technologies and strategies to deliver customized solutions that are specific to your needs.
For researchers or drug discovery groups needing a humanized target, we consult with you and JAX genetics experts to help design and generate your ideal model. We can further validate both target engagement and downstream signaling as part of model phenotyping services and pharmacokinetics analysis.
JAX's Transgenic Model Generation Service includes pronuclear microinjection of your transgenic DNA construct (plasmid or BAC) into pronuclear embryos that are then transferred into pseudopregnant females. Pups are then genotyped to identify founder mice (three, on average) and shipped at six weeks of age. JAX routinely achieves a 90% success rate for transgenic mouse generation, so your model will be ready in 12-14 weeks. Breeding to germline transmission can be performed through our Breeding Services.
Typically, six-week-old founder transgenic mice will be available to ship to your facility or to the JAX Breeding Services vivarium, 12-14 weeks after we receive your DNA. JAX will deliver all the animals with confirmed genetic modifications resulting from the microinjection. A majority of our customers don’t quarantine or rederive mice received from JAX, and import them directly into their facility.
In order to meet the need for blastocyst injections of modified ES cells, we offer microinjection services of your ES cell clone into appropriate host blastocysts. Following microinjection, embryos are transferred to host females, producing chimeric founder mice carrying the new genetic modification. Chimeric mice will be ready to breed in 13-15 weeks. Subsequent breeding to produce mice derived from the targeted ES cells (germline transmission) can be performed in your facility or through JAX Breeding Services
Once clones arrive, we will karyotype them to determine whether there are major chromosomal abnormalities as part of our full-service offering. Once this is complete, the mES cells will be microinjected into host blastocysts and transferred into pseudopregnant females. Chimeric pups will be identified by coat color and delivered at six weeks of age. Chimeric mice generated are of high health status so many customers choose to import them directly into their facility without quarantining or rederiving.
Our CRISPR/CAS9 mouse model creation service offers a customized package delivering mouse models for your research or drug discovery journey.
CRISPR technology enables the creation of more precise models (with no extraneous sequence) giving us the ability to create the ideal model for your research or drug discovery needs in just a few months.
Cre-lox technology is one of the most widely used technologies for creating conditional mutations. With JAX, you have access to one of the world’s largest collections of tissue specific and inducible cre strains, which can be bred with your floxed stain to produce the conditional mutation of interest.
JAX offers several other services that still tailor models to your specific needs including, but not limited to:
Using a wide variety of model generation technologies, our expert mouse geneticists and team of subject matter experts will work with you to design a model that will best achieve your research or drug discovery needs. Our capabilities include:
While working with JAX, we will provide you with regular updates along the way, including a founder packet and N1 data packet. These packets will be discussed with you by your JAX partner. Your mice are guaranteed to be germline-competent and genetically validated.
You can then have your mice shipped right to your institution’s door, or utilize JAX’s expertise further to have your colony expanded, preserved, characterized, tested, and analyzed. We invite you to explore the variety of breeding and in vivo preclinical testing and efficacy services that offer additional solutions beyond model generation.
With JAX, you can turn your newly created genetically modified JAX mouse into a validated model through expression analysis and/or phenotypic characterization.
Our experts are ready to help. Our team will work with you to find the best plan for your goals. We can provide the tools to achieve reliable results, and we can perform services for you to boost your productivity and unlock operational resources. Either way, we are here to help.Get Started