Guide: Comprehensive Evaluation of Human Immune System Reconstitution in NSG™ and NSG™-SGM3 Mouse Models

 

NSG™ and NSG™-SGM3 mice engrafted with allogeneic human tumors represent a valuable preclinical testing platform for immuno-oncology. Here we employ four 14-color flow cytometry panels to perform a comprehensive and detailed analysis of the entire immune system. Our results indicate that the triple transgenic NSG™-SGM3 mice exhibit a more completely humanized immune system as compared to NSG™ mice, with specific improvements in the distribution of T-cell subsets and overall representation of the myeloid lineage.

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