Jackson Laboratory

NSG™ Variants Portfolio

NSG™ mouse model variants are the most highly immunodeficient
mice and the models of choice for cancer xenograft modeling, stem
cell biology, humanized mice, and infectious disease research.

Comparison of NSG™ Mouse Model Variants

Name & Stock Number	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (005557)	NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (013062)	NOD.Cg-Rag1^tm1Mom Il2rg^tm1Wjl/SzJ (007799)
Branded or common name	NSG™(branded name), NOD scid gamma	NSGS, NOD scid gamma Il3- GM-SF (NSG-SGM3)	NRG, NOD Rag gamma
Mature B cells	Absent	Absent	Absent
Mature T cells	Absent	Absent	Absent
Dendritic cells	Defective	Defective	Defective
Macrophages	Defective	Defective	Defective
Natural killer cells	Absent	Absent	Absent
Complement	Absent	Absent	Absent
Leakiness	Negligible	Absent	Absent
Irradiation tolerance	Low	Low	High
Lymphoma incidence	Low	Low	Low
Benefits	Engrafts the widest range of solid and hematological cancers, including ALL and AML Most sensitive host for cancer stem cells when compared to NOD scid or nude mice Longer lifespan than NOD scid; supports long-term engraftment studies and capabilities; >89 weeks median	Increased CD4+ FoxP3+ regulatory T cell population after CD34+ humanization Enhances human myelopoiesis and terminal differentiation after CD34+ humanization Increased efficiency of engrafting human acute myeloid leukemia (AML)	Long-term multilineage hematopoeitic stem cell repopulation similar to NSG mice Engrafts human PBMC without irradiation similar to NSG Engrafts a wide range if solid and hematological cancers
Considerations	No thymic lymphomas, can be used for long & short-term experiments Sensitive to irradiation	Compromised human stem cell regeneration Suppression of human erythropoiesis Reduction of human B-lymphopoiesis	No thymic lymphomas, can be used for long & short-term experiments Requires higher dose of irradiation to obtain human HSC engraftment
References	Ishikawa et al. 2005; Shultz et al. 2005	Nicolini et al. 2004; Wunderlich et al. 2010; Billerbeck et al. 2015	Pearson et al. 2008; Brehm et al. 2010; Maykel et al. 2014

(continued)

Name & Stock Number	NSG-HLA-A2.1 (009617) NSG-HLA-A2/HHD (014570)	DR1 (012479) DR4 (017637)	NSG B2m (010636) NSG-(K^bD^b)^null(023848)
Branded or common name	HLA Class I-A2 Transgenics	HLA Class II Transgenics	MHC Class I-null NSG™
Mature B cells	Absent	Absent	Absent
Mature T cells	Absent	Absent	Absent
Dendritic cells	Defective	Defective	Defective
Macrophages	Defective	Defective	Defective
Natural killer cells	Absent	Absent	Absent
Complement	Absent	Absent	Absent
Leakiness	Negligible	Negligible	Negligible
Irradiation tolerance	Low	Low	Low
Lymphoma incidence	Low	Low	Low
Benefits	High engraftment of HLA-A2-restricted immune cells after CD34+ humanization Enable functional CD4+ T cell responses to viral infection after CD34+ humanization (014570)	Useful for transplantation studies in the absence of xeno-GVHD Develop allo-GVHD post-engraftmentof DR4-negative CD4+ T cells (017637)	Resistant to xeno-GVHD (010636) Attenuated xeno-GVHD development post-hPBMC transplantation (023848) High hCD45+ cell engraftment (023848) Useful for studying mechanisms for xeno-GVHD
Considerations	No thymic lymphomas - can be used for long-term experiments Sensitive to irradiation	Reduced CD45+ cell engraftment compared to NSG Higher proportion of mice with no CD45+ cell engraftment as compared to NSG (017637)	Reduced survival post hCD34+ cell transplantation compared to NSG mice (023848)
References	Shultz et al. 2010	Covassin et al. 2011	Covassin et al. 2013

(continued)

Name & Stock Number	MHC I/II KO (025216)	IL-15 (030890)
Branded or common name	Class 1/Class 2 Knockout	NSG-IL15
Mature B cells	Absent	Absent
Mature T cells	Absent	Absent
Dendritic cells	Defective	Defective
Macrophages	Defective	Defective
Natural killer cells	Absent	Absent
Complement	Absent	Absent
Leakiness	Negligible	Negligible
Irradiation tolerance	Low	Low
Lymphoma incidence	Low	Low
Benefits	Most resistant to xeno-GVHD of all NSG variants Extended human IgG half-life compared to B2m knockouts (10636)	Increased abundance and function of human NK cells following CD34-humanization
Considerations	Resistance to xeno-GVHD associated with reduced CD45+ cell expansion in vivo Radiation sensitive	Sensitive to irradiation
References	Brehm et al. 2018	Brehm et al. 2018
Variant Research area	Options currently are: Immunology HIV and infectious disease Cancer Transplantation research Diabetes	Options currently are: Immunology Immuno-oncology Cancer