The transgenic NSG-Tg(Hu-IL15) mice express human IL15 and combined with the highly immunodeficient NOD scid gamma (NSG) mouse. Expression of human IL15 enhances the development of human NK cells in immunodeficient mice engrafted with human stem cells. This strain may be useful for studying immunotherapies targeting NK cells and NK cell function in tumorogenesis.
Dr. Leonard D. Shultz, The Jackson Laboratory
Contact Technical Support
|Allele Type||Gene Symbol||Gene Name|
|Spontaneous||Prkdc||protein kinase, DNA activated, catalytic polypeptide|
|Allele Type||Gene Symbol||Gene Name|
|Targeted (Null/Knockout)||Il2rg||interleukin 2 receptor, gamma chain|
|Transgenic (Inserted expressed sequence, Humanized sequence)|
NSG-Tg(Hu-IL15) mutant mice combine the features of the severe combined immune deficiency mutation or scid (B and T cell deficiency) and IL2 receptor gamma chain deficiency (NK cell deficiency) with expression of the human IL15 gene on the NOD/ShiLtJ background. Mice homozygous for the three mutations are viable, normal in size and do not display any gross physical or behavioral abnormalities; survival is similar to NSG mice. Hemizygous NSG-Tg(Hu-IL15) mice express a physiological level of human IL15 (7.1 +/- 0.3 pg/ml). Mice irradiated and injected with CD34+ human stems cells (HSC) develop significantly higher levels of functional human CD56+ NK cells in peripheral blood, spleen and bone marrow than NSG controls. This strain may be useful for studying immunotherapies targeting NK cells and NK cell function in tumorogenesis.
The transgenic construct consists of a 200 kbp human BAC RP11-620F30 containing the complete human IL15 (interleukin 15) gene. No other human genes are present in the BAC. The transgene was microinjected into fertilized NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (Stock No. Stock No. 005557) oocytes. Founder mice were bred to NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG - Stock No. 005557) and the strain was maintained on the NSG background.
|Allele Name||severe combined immunodeficiency|
|Allele Synonym(s)||Prkdcscid; severe combined immunodeficiency|
|Gene Symbol and Name||Prkdc, protein kinase, DNA activated, catalytic polypeptide|
|Gene Synonym(s)||AI326420; DNA-PK; DNA-PKcs; DNAPDcs; DNPK1; DOXNPH; doxorubicin nephropathy; DNAPK; expressed sequence AU019811; dxnph; dxnph; scid; IMD26; HYRC; HYRC1; p350; slip; XRCC7; severe combined immunodeficiency; expressed sequence AI326420; AU019811; DNA-PKC; DNAPKc|
|Site of Expression||T and B lymphocytes.|
|Strain of Origin||C.B-17|
|Molecular Note||A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon.|
|Allele Name||targeted mutation 1, Warren J Leonard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Il2rgtm1Wjl; targeted mutation 1, Warren J Leonard|
|Gene Symbol and Name||Il2rg, interleukin 2 receptor, gamma chain|
|Gene Synonym(s)||common cytokine receptor gamma chain; CIDX; IMD4; gc; IL-2RG; P64; [g]c; SCIDX; SCIDX1; common gamma chain; gamma C receptor; gamma(c); CD132; Ab2-183; Cd132; gammaC|
|Site of Expression||Primarily lymphoid cells.|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.|
|Mutations Made By|| |
Dr. Warren Leonard, NHLBI, NIH
|Allele Name||transgene insertion 1, Leonard Shultz|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||Tg(IL15)1Sz; transgene insertion 1, Leonard Shultz|
|Gene Symbol and Name||Tg(IL15)1Sz, transgene insertion 1, Leonard Shultz|
|Strain of Origin||NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ|
|Molecular Note||The transgenic construct consists of a 200 kbp human BAC RP11-620F30 containing the complete human IL15 (interleukin 15) gene. Mice express a physiological level of human IL15 (7.1 +/- 0.3 pg/ml).|
When maintaining a live colony, these mice are bred as homozygous for all three alleles (Il2rg is X-linked). Specific pathogen-free (SPF) conditions are recommended.
When using the NSG IL15 Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #030890 in your Materials and Methods section.
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.