NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(IL15)1Sz/SzJ

Stock number: 030890
Product name: NSG-Tg(Hu-IL15)

Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

The transgenic NSG-Tg(Hu-IL15) mice express human IL15 and combined with the highly immunodeficient NOD scid gamma (NSG) mouse. Expression of human IL15 enhances the development of human NK cells in immunodeficient mice engrafted with human stem cells. This strain may be useful for studying immunotherapies targeting NK cells and NK cell function in tumorigenesis.

To order this model with CD34+ or PBMC engraftment, please visit our Humanized Mice page.

Detailed description

NSG-Tg(Hu-IL15) mutant mice combine the features of the severe combined immune deficiency mutation or scid (B and T cell deficiency) and IL2 receptor gamma chain deficiency (NK cell deficiency) with expression of the human IL15 gene on the NOD/ShiLtJ background. Mice homozygous for the three mutations are viable, normal in size and do not display any gross physical or behavioral abnormalities; survival is similar to NSG mice. Hemizygous NSG-Tg(Hu-IL15) mice express a physiological level of human IL15 (7.1 +/- 0.3 pg/ml). Mice irradiated and injected with CD34+ human stems cells (HSC) develop significantly higher levels of functional human CD56+ NK cells in peripheral blood, spleen and bone marrow than NSG controls. This strain may be useful for studying immunotherapies targeting NK cells and NK cell function in tumorigenesis.

Development

The transgenic construct consists of a 200 kbp human BAC RP11-620F3 containing the complete human IL15 (interleukin 15) gene. No other human genes are present in the BAC. The transgene was microinjected into fertilized NSG oocytes (Stock No. 005557: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ). Founder mice were bred to NSG and the strain was thereafter maintained on the NSG background.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Il2rg^tm1Wjl
Name: targeted mutation 1, Warren J Leonard
MGI accession ID: MGI:1857455
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il2rg
Marker name: interleukin 2 receptor, gamma chain
Chromosome: X
Strain of origin: 129S4/SvJae
Site of expression: Primarily lymphoid cells.
Molecular note: A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.

Allele

Symbol: Tg(IL15)1Sz
Name: transgene insertion 1, Leonard Shultz
MGI accession ID: MGI:6201746
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(IL15)1Sz
Marker name: transgene insertion 1, Leonard Shultz
Chromosome: UN
Strain of origin: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ
Site of expression: IL15 has widespread expression in a large number of cells and tissues.

Molecular note: The transgenic construct consists of a 200 kbp human BAC RP11-620F30 containing the complete human IL15 (interleukin 15) gene. Mice express a physiological level of human IL15 (7.1 +/- 0.3 pg/ml).