NBSGW mice support engraftment of human hematopoietic stem cells without irradiation. They are suitable for use in applications related to xenotransplantation.
James A. Thomson, The University of Wisconsin
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Kit | KIT proto-oncogene receptor tyrosine kinase |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il2rg | interleukin 2 receptor, gamma chain |
Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Tyr | tyrosinase |
Xenograft NBSGW mice exhibit enhanced human hematopoietic chimerism in peripheral blood, bone marrow, and spleen when compared to non-irradiated engrafted NSG mice, with a level of chimerism similar to that seen in irradiated NSG mice. Chimerism in the peripheral blood of engrafted NBSGW mice was found to be 9-fold higher than chimerism exhibited by engrafted NSG mice. Serial transplantation of human hematopoietic stem cells is also supported with evidence of secondary graft expansion in the secondary recipients. Mice are mottled grey and white coat color due to the homozygous wildtype Tyr (tyrosinase) allele. While establishing the strain, mice that were homozygous for the wildtype Tyr (tyrosinase) allele were selected for further breeding to eliminate the albino coat color from the colony (allows easier visual phenotyping of KitW-41J allele). Mice that are homozygous for all three of these mutant alleles are viable and fertile.
These NBSGW mice may be expected to have a similar phenotype as the NSGW41 model (Stock No. 026497).
Heterozygous C57BL/6J-KitW-41J/J (Stock No. 000119) mice were intercrossed to generate homozygotes. These homozygous C57BL/6J-KitW-41J/J mice were then crossed to NSG mice, NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (Stock No. 005557) to produce mice heterozygous for the following alleles: KitW-41J, Prkdcscid, Il2rgtm1Wjl, and the NOD/ShiLtJ Sirpa variant. The mice were intercrossed to homozygosity for all four alleles. The mice were then crossed to NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (Stock No. 005557) for 5 generations. Mice that were homozygous for the wildtype Tyr (tyrosinase) allele were selected for further breeding to eliminate the albino coat color from the colony (allows easier visual phenotyping of KitW-41J allele).
Allele Name | dominant spotting 41 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | KitW41; W41; W-41 |
Gene Symbol and Name | Kit, KIT proto-oncogene receptor tyrosine kinase |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 5 |
Molecular Note | Comparison of the coding sequence of this allele with normal c-kit indicated a G-to-A point mutation in the kinase domain at nucleotide 2519 that results in a valine to methionine substitution at amino acid 831 (p.V831M). |
Allele Name | severe combined immunodeficiency |
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Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | targeted mutation 1, Warren J Leonard |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | [KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull |
Gene Symbol and Name | Il2rg, interleukin 2 receptor, gamma chain |
Gene Synonym(s) | |
Site of Expression | Primarily lymphoid cells. |
Strain of Origin | 129S4/SvJae |
Chromosome | X |
Molecular Note | A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein. |
Mutations Made By | Dr. Warren Leonard, NHLBI, NIH |
When maintaining a live colony, these mice can be bred as homozygotes for the KitW-41J, Prkdcscid, Il2rgtm1Wjl alleles (males would be hemizygous for the X-linked Il2rgtm1Wjl).
When using the NBSGW mouse strain in a publication, please cite the originating article(s) and include JAX stock #026622 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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