Overview

Also Known As:NBSGW

NBSGW mice support engraftment of human hematopoietic stem cells without irradiation. They are suitable for use in applications related to xenotransplantation.

Donating Investigator

James A. Thomson, The University of Wisconsin

Genetic overview

Genetic Background	Generation
	Contact Technical Support (2020-11-05 00:00:00)

Kit^W-41J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Kit	KIT proto-oncogene receptor tyrosine kinase

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

Allele Type	Gene Symbol	Gene Name
Not Applicable	Tyr	tyrosinase

Research Applications

Mouse/Human Gene Homologs
Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Dermatology Research
Reproductive Biology Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$248.32 Domestic price for male 3-week

View Price List

Details

Detailed Description

Xenograft NBSGW mice exhibit enhanced human hematopoietic chimerism in peripheral blood, bone marrow, and spleen when compared to non-irradiated engrafted NSG mice, with a level of chimerism similar to that seen in irradiated NSG mice. Chimerism in the peripheral blood of engrafted NBSGW mice was found to be 9-fold higher than chimerism exhibited by engrafted NSG mice. Serial transplantation of human hematopoietic stem cells is also supported with evidence of secondary graft expansion in the secondary recipients. Mice are mottled grey and white coat color due to the homozygous wildtype Tyr (tyrosinase) allele. While establishing the strain, mice that were homozygous for the wildtype Tyr (tyrosinase) allele were selected for further breeding to eliminate the albino coat color from the colony (allows easier visual phenotyping of Kit^W-41J allele). Mice that are homozygous for all three of these mutant alleles are viable and fertile.

These NBSGW mice may be expected to have a similar phenotype as the NSGW41 model (Stock No. 026497).

Development

Heterozygous C57BL/6J-Kit^W-41J/J (Stock No. 000119) mice were intercrossed to generate homozygotes. These homozygous C57BL/6J-Kit^W-41J/J mice were then crossed to NSG mice, NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (Stock No. 005557) to produce mice heterozygous for the following alleles: Kit^W-41J, Prkdc^scid, Il2rg^tm1Wjl, and the NOD/ShiLtJ Sirpa variant. The mice were intercrossed to homozygosity for all four alleles. The mice were then crossed to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (Stock No. 005557) for 5 generations. Mice that were homozygous for the wildtype Tyr (tyrosinase) allele were selected for further breeding to eliminate the albino coat color from the colony (allows easier visual phenotyping of Kit^W-41J allele).

Control Suggestions

Approximate Controls

005557 NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ

Additional Information

Considerations for Choosing Controls

Selected References

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61PubMed: 7274658 MGI: J:6571
McIntosh BE; Brown ME; Duffin BM; Maufort JP; Vereide DT; Slukvin II; Thomson JA. 2015. Nonirradiated NOD,B6.SCID Il2rgamma(-/-)Kit(W41/W41) (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells. Stem Cell Reports 4(2):171-80PubMed: 25601207 MGI: J:218738
Shultz LD; Lyons BL; Burzenski LM; Gott B; Chen X; Chaleff S; Kotb M; Gillies SD; King M; Mangada J; Greiner DL; Handgretinger R. 2005. Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol 174(10):6477-89PubMed: 15879151 MGI: J:109833

View All References

Genetics

Kit^W-41J

Allele Symbol: Kit^W-41J

Allele Name	dominant spotting 41 Jackson
Allele Type	Spontaneous
Allele Synonym(s)	Kit^W41; W⁴¹; W-41
Gene Symbol and Name	Kit, KIT proto-oncogene receptor tyrosine kinase
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	5
Molecular Note	Comparison of the coding sequence of this allele with normal c-kit indicated a G-to-A point mutation in the kinase domain at nucleotide 2519 that results in a valine to methionine substitution at amino acid 831 (p.V831M).

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Tyr⁺

Allele Symbol: Tyr⁺

Allele Name	wild type
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	Tyr, tyrosinase
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	7

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Tyr⁺ related

Mouse/Human Gene Homologs
- albinism, tyrosine negative
  - wildtype
Dermatology Research
- Color and White Spotting Defects
  - oculocutaneous albinism, type I

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Genotype: Kit^W-41J related

Reproductive Biology Research
- Fertility Defects
- Gonadal Tumors
  - ovarian
- Developmental Defects Affecting Gonads
  - germ cell deficient
Cancer Research
- Increased Tumor Incidence
  - Gonadal Tumors: ovarian
  - Gonadal Tumors
- Growth Factors/Receptors/Cytokines
- Oncogenes
Mouse/Human Gene Homologs
- piebaldism
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - Mast Cell Deficiency
Endocrine Deficiency Research
- Skin Defects
- Bone/Bone Marrow Defects
- Gonad Defects
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
Neurobiology Research
- Receptor Defects
- Hearing Defects
Sensorineural Research
- Hearing Defects
Dermatology Research
- Color and White Spotting Defects
Developmental Biology Research
- Neural Crest Defects

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
  - xenograft/transplant host
Research Tools
- Cancer Research
  - xenograft/transplant host
- Immunology, Inflammation and Autoimmunity Research
Hematological Research
- Immunological Defects
  - B and T cell deficiency

Genotype: Il2rg^tm1Wjl related

Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Mammalian Phenotype Terms by Genotype

References

Geissler EN; McFarland EC; Russell ES. 1981. Analysis of pleiotropism at the dominant white-spotting (W) locus of the house mouse: a description of ten new W alleles. Genetics 97(2):337-61PubMed: 7274658 MGI: J:6571
McIntosh BE; Brown ME; Duffin BM; Maufort JP; Vereide DT; Slukvin II; Thomson JA. 2015. Nonirradiated NOD,B6.SCID Il2rgamma(-/-)Kit(W41/W41) (NBSGW) Mice Support Multilineage Engraftment of Human Hematopoietic Cells. Stem Cell Reports 4(2):171-80PubMed: 25601207 MGI: J:218738
Shultz LD; Lyons BL; Burzenski LM; Gott B; Chen X; Chaleff S; Kotb M; Gillies SD; King M; Mangada J; Greiner DL; Handgretinger R. 2005. Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol 174(10):6477-89PubMed: 15879151 MGI: J:109833

Additional - Il2rg^tm1Wjl related

Additional - Kit^W-41J related

Additional - Prkdc^scid related

Additional - Tyr⁺ related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Pyrosequencing:Tyr
- Pyrosequencing:Kit
- End Point Analysis:Tyr c-EP
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- End Point Analysis:Kit-EP
- End Point Analysis:Tyr alternative 1
- End Point Analysis:Tyr alternative 1
- Sanger sequencing:Tyr-SEQ
- Genotyping resources and troubleshooting
Breeding Considerations

This strain is a good breeder.

When maintaining a live colony, these mice can be bred as homozygotes for the Kit^W-41J, Prkdc^scid, Il2rg^tm1Wjl alleles (males would be hemizygous for the X-linked Il2rg^tm1Wjl).
- Additional Breeding and Husbandry Support
Mating System
- Females homozygous for the Kit^W-41J, Prkdc^scid, Il2rg^tm1Wjl alleles x Males homozygous for the Kit^W-41J, Prkdc^scid, and hemizygous for the Il2rg^tm1Wjl alleles
Appearance
- Mice are mottled grey and white.
Citation
When using the NBSGW mouse strain in a publication, please cite the originating article(s) and include JAX stock #026622 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX27 (Maximum)

Pricing & Availability

Readily Available

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Please inquire

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by non-profits requires a Material Transfer Agreement (MTA) and for-profit entities require a license.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NBSGW

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

KitW-41J

Allele Symbol: KitW-41J

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Tyr+

Allele Symbol: Tyr+

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Tyr+ related

Genotype: Prkdcscid related

Genotype: KitW-41J related

Genotype: Il2rgtm1Wjl related

Mammalian Phenotype Terms by Genotype

References

Additional - Il2rgtm1Wjl related

Additional - KitW-41J related

Additional - Prkdcscid related

Additional - Tyr+ related

Genotyping Protocols

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Kit^W-41J

Allele Symbol: Kit^W-41J

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Tyr⁺

Allele Symbol: Tyr⁺

Genotype: Tyr⁺ related

Genotype: Prkdc^scid related

Genotype: Kit^W-41J related

Genotype: Il2rg^tm1Wjl related

Additional - Il2rg^tm1Wjl related

Additional - Kit^W-41J related

Additional - Prkdc^scid related

Additional - Tyr⁺ related