Overview

Also Known As:NSGS, NSG-SGM3, NOD-scid IL2Rgnull-3/GM/SF, NSG-Triples [NSG-T], NSG-SGM3

The triple transgenic NSG-SGM3 (NSGS) mice expressing human IL3, GM-CSF (CSF2) and SCF (KITLG) combine the features of the highly immunodeficient NOD scid gamma (NSG) mouse with cytokines that support the stable engraftment of myeloid lineages and regulatory T cell populations. As a result, these NSG-SGM3 mice allow superior engraftment of diverse hematopoietic lineages and primary AML samples than other models and are useful for immuno-oncology, immunology and infectious disease studies.

Donating Investigator

James C Mulloy, Cincinnati Children's Hospital Medical Center

Genetic overview

Genetic Background	Generation
	Contact Technical Support (2018-07-27 00:00:00)

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

Tg(CMV-IL3,CSF2,KITLG)1Eav

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Virology Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$249.90 Domestic price for male 3-week

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Volume Pricing Available!

for select shipping destinations

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Details

Detailed Description

The triple transgenic NSG-SGM3 (NSGS) mice are NOD.scid.Il2Rγc^null ("NSG") animals with the SGM3 coinjected transgenes, each with a human cytomegalovirus promoter/enhancer sequence driving expression of the human interleukin-3 gene (IL3; IL-3), the human granulocyte/macrophage-stimulating factor gene (CSF2; GM-CSF), or the human Steel factor gene (KITLG; SCF or SF).

NSG-SGM3 mice combine the features of the highly immunodeficient NSG mouse with cytokines (IL3, GM-CSF and SCF) that support the stable engraftment of myeloid lineages and regulatory T cell populations. NSG-SGM3 mice allow superior engraftment of diverse hematopoietic lineages and primary AML samples than other models and are useful for immuno-oncology, immunology and infectious disease studies.

These NSG-SGM3 mice constitutively produce 2-4 ng/ml serum levels of human IL-3, GM-CSF, and SF.

Similar to other immunodeficient strains, maintaining NSG-SGM3 mice in high health status (specific pathogen-free) vivaria promotes overall colony health. If homozygous NSG-SGM3 animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.

View our Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, flow cytometry data and categorized, up-to-date references.

Development

Three separate transgenes were designed each carrying either the human interleukin-3 gene (IL3; IL-3), the human granulocyte/macrophage-stimulating factor gene (CSF2; GM-CSF), or the human Steel factor gene (KITLG; SCF or SF). Expression of each gene is driven by a human cytomegalovirus promoter/enhancer sequence, and is followed by a human growth hormone cassette and a polyadenylation (polyA) sequence. The transgenes were microinjected into fertilized C57BL/6xC3H/HeN oocytes. The resulting founders, carrying all three transgenes (3GS) were backcrossed to BALB/c-scid/scid mice for several generations and subsequently backcrossed to NOD.CB17-Prkdc^scid mice for at least 11 generations. These mice were bred to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl mice, and were then interbred until all offspring were homozygous for 3GS and the IL2rg targeted mutation. Upon arrival at The Jackson Laboratory, transgenic mice were bred to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Stock No. 005557) for one generation to establish the colony. Thereafter, females homozygous for Prkdc^scid, homozygous for Il2rg^tm1Wjl and homozygous for the 3GS transgenes were bred with males homozygous for Prkdc^scid, hemizygous for the X-linked Il2rg^tm1Wjl and homozygous for the 3GS transgenes.

Expression Data

Expressed Gene	KITLG, KIT ligand, human
Expressed Gene	CSF2, colony stimulating factor 2, human
Expressed Gene	IL3, interleukin 3, human
Site of Expression

Control Suggestions

Additional Information

Considerations for Choosing Controls

Selected References

Billerbeck E; Barry WT; Mu K; Dorner M; Rice CM; Ploss A. 2011. Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor-, granulocyte-macrophage colony-stimulating factor-, and interleukin-3-expressing NOD-SCID IL2R{gamma}null humanized mice. Blood 117(11):3076-86PubMed: 21252091 MGI: J:170510
Bryce PJ; Falahati R; Kenney LL; Leung J; Bebbington C; Tomasevic N; Krier RA; Hsu CL; Shultz LD; Greiner DL; Brehm MA. 2016. Humanized mouse model of mast cell-mediated passive cutaneous anaphylaxis and passive systemic anaphylaxis. J Allergy Clin Immunol 138(3):769-779PubMed: 27139822 MGI: J:231799
Coughlan AM; Harmon C; Whelan S; O'Brien EC; O'Reilly VP; Crotty P; Kelly P; Ryan M; Hickey FB; O'Farrelly C; Little MA. 2016. Myeloid Engraftment in Humanized Mice: Impact of Granulocyte-Colony Stimulating Factor Treatment and Transgenic Mouse Strain. Stem Cells Dev 25(7):530-41PubMed: 26879149 MGI: J:230785
Wunderlich M; Chou FS; Link KA; Mizukawa B; Perry RL; Carroll M; Mulloy JC. 2010. AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3. Leukemia 24(10):1785-8PubMed: 20686503 MGI: J:166359

View All References

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Tg(CMV-IL3,CSF2,KITLG)1Eav

Allele Symbol: Tg(CMV-IL3,CSF2,KITLG)1Eav

Allele Name	transgene insertion 1, CJ Eaves
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	3/GM/SF; SGM3; Tg(hSCF/hGM-CSF/hIL3
Gene Symbol and Name	Tg(CMV-IL3,CSF2,KITLG)1Eav, transgene insertion 1, CJ Eaves
Gene Synonym(s)
Promoter	CMV, cytomegalovirus, human
Expressed Gene	KITLG, KIT ligand, human
Expressed Gene	CSF2, colony stimulating factor 2, human
Expressed Gene	IL3, interleukin 3, human
Strain of Origin	(C57BL/6 x C3H/HeN)F1
Chromosome	UN
Molecular Note	cDNAs encoding human interleukin 3, colony stimulating factor 2 (granulocyte-macrophage) and kit ligand were independently ligated into expression cassettes in which the cDNA resides downstream of the cytomegalovirus (CMV) promoter/enhancer sequence and is followed by human growth factor intronic and polyadenylation sequences. A transgenic mouse that was shown by ELISA to have serum levels of all three growth factors at ng/ml levels was used to establish a line with the same phenotype.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Il2rg^tm1Wjl related

Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
Cancer Research
- Growth Factors/Receptors/Cytokines

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Mammalian Phenotype Terms by Genotype

Phenotype Information

Body Weight Information - JAX^® Mice Strain - NOD.Cg-Prkdc^scidIl2rg^tm1WjlTg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ

References

Billerbeck E; Barry WT; Mu K; Dorner M; Rice CM; Ploss A. 2011. Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor-, granulocyte-macrophage colony-stimulating factor-, and interleukin-3-expressing NOD-SCID IL2R{gamma}null humanized mice. Blood 117(11):3076-86PubMed: 21252091 MGI: J:170510
Bryce PJ; Falahati R; Kenney LL; Leung J; Bebbington C; Tomasevic N; Krier RA; Hsu CL; Shultz LD; Greiner DL; Brehm MA. 2016. Humanized mouse model of mast cell-mediated passive cutaneous anaphylaxis and passive systemic anaphylaxis. J Allergy Clin Immunol 138(3):769-779PubMed: 27139822 MGI: J:231799
Coughlan AM; Harmon C; Whelan S; O'Brien EC; O'Reilly VP; Crotty P; Kelly P; Ryan M; Hickey FB; O'Farrelly C; Little MA. 2016. Myeloid Engraftment in Humanized Mice: Impact of Granulocyte-Colony Stimulating Factor Treatment and Transgenic Mouse Strain. Stem Cells Dev 25(7):530-41PubMed: 26879149 MGI: J:230785
Wunderlich M; Chou FS; Link KA; Mizukawa B; Perry RL; Carroll M; Mulloy JC. 2010. AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3. Leukemia 24(10):1785-8PubMed: 20686503 MGI: J:166359

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Additional - Tg(CMV-IL3,CSF2,KITLG)1Eav related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- QPCR:TG(CSF2)
- QPCR:TG(IL3)
- QPCR:TG(KITLG)
- Standard PCR:Tg(KITLG)3Ygy
- Standard PCR:Il2rg
- Standard PCR:Tg(CSF2)2Ygy
- Standard PCR:Tg(IL3)1Ygy
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

This strain is a good breeder.

When maintaining a live colony, females homozygous for scid (Prkdc^scid), homozygous for the X-linked Il2Rγc^null (Il2rg^tm1Wjl) and homozygous for the SGM3 transgenes (Tg(CMV-IL3,CSF2,KITLG)1Eav) may be bred with males homozygous for scid, hemizygous for the X-linked Il2Rγc^null and homozygous for the SGM3 transgenes.

Homozygous NSG-SGM3 mice are immunodeficient. As such, and similar to other immunodeficient strains, maintaining NSG-SGM3 mice in high health status (specific pathogen-free) vivaria promotes overall colony health. If homozygous NSG-SGM3 animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health. Also see additional NSG housing information.
- Additional Breeding and Husbandry Support
Mating System
- See "Breeding Considerations"
Citation
When using the NSG-SGM3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #013062 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

RB16 (Maximum)

EM01 (Maximum)

RB08 (Maximum)

Pricing & Availability

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Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

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Volume Pricing Details

Domestic Commercial & For-Profit

QUANTITY	Volume Pricing
Volume pricing is not available for Domestic Commercial & For-Profit.

Domestic Not-For-Profit & Academic

QUANTITY	Volume Pricing
Volume pricing is not available for Domestic Not-For-Profit & Academic.

International Commercial & For-Profit

QUANTITY	Volume Pricing
Volume pricing is not available for International Commercial & For-Profit.

International Not-For-Profit & Academic

QUANTITY	Volume Pricing
Volume pricing is not available for International Not-For-Profit & Academic.

Volume Pricing Program
Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached
Sexes: Sexes of the same strain may be combined to reach minimum quantity levels to receive the volume pricing
Shipment: All shipping destinations qualify

Cryorecovery - Pricing

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Research institutions require an MTA, companies require a (no fee) commercial use agreement prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NSGS, NSG-SGM3, NOD-scid IL2Rgnull-3/GM/SF, NSG-Triples [NSG-T], NSG-SGM3

Donating Investigator

Genetic overview

Research Applications

Base Price

Volume Pricing Available!

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Tg(CMV-IL3,CSF2,KITLG)1Eav

Allele Symbol: Tg(CMV-IL3,CSF2,KITLG)1Eav

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Il2rgtm1Wjl related

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

Phenotype Information

References

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Additional - Tg(CMV-IL3,CSF2,KITLG)1Eav related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Volume Pricing Details

Domestic Commercial & For-Profit

Domestic Not-For-Profit & Academic

International Commercial & For-Profit

International Not-For-Profit & Academic

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Genotype: Il2rg^tm1Wjl related

Genotype: Prkdc^scid related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related