These mice are most often referred to using their branded name "NSG™" and are extremely immunodeficient. The mice carry two mutations on the NOD/ShiLtJ genetic background; severe combined immune deficiency (scid) and a complete null allele of the IL2 receptor common gamma chain (IL2rgnull). The scid mutation is in the DNA repair complex protein Prkdc and renders the mice B and T cell deficient. The IL2rgnull mutation prevents cytokine signaling through multiple receptors, leading to a deficiency in functional NK cells. The severe immunodeficiency allows the mice to be humanized by engraftment of human CD34+ hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), patient derived xenografts (PDX), or adult stem cells and tissues. The immunodeficient NSG mice enable research in human immune function, infectious disease, diabetes, oncology, and stem cell biology.
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Dr. Leonard D. Shultz, The Jackson Laboratory
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|Allele Type||Gene Symbol||Gene Name|
|Spontaneous||Prkdc||protein kinase, DNA activated, catalytic polypeptide|
|Allele Type||Gene Symbol||Gene Name|
|Targeted (Null/Knockout)||Il2rg||interleukin 2 receptor, gamma chain|
Like its parental strain, NOD/ShiLtJ (Stock No. 001976), NSG mice exhibit a progressive hearing loss that is already severe by three months of age.
The NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice, most often known by their branded name, NOD scid gamma (NSG™), do not express the Prkdc gene nor the X-linked Il2rg gene. NSG mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological examination of lymphoid tissues reveals absence of lymphoid cells and some cystic structures in the thymus, an absence of follicles in the spleen and markedly diminished celluarity of lymph nodes. NSG mice are deficient in mature lymphocytes, serum Ig is not detectable and natural killer (NK) cell cytotoxic activity is extremely low. These mice are resistant to lymphoma development even after sublethal irradiation treatment. These mutant mice have been shown to readily support engraftment of human CD34+ hematopoietic stem cells and represent a superior, long-lived model suitable for studies employing xenotransplantation strategies. NSG mice are highly sensitive to the diabetogenic effects of streptozotocin (STZ).
Kinked tail, head tilt, circling, and hind limb lameness are observed at low frequency. These conditions are very infrequent (<0.01% incidence) in young, experimentally naïve mice. Incidence is estimated at <0.5% in older mice used for humanization and other studies.
The NSG-STZ preclinical model for pancreatic islet transplantation is now available from JAX® Services.
Please note that the NSG carries the true null interleukin-2 receptor gamma chain mutation and should not be confused with other strains that express a truncated interleukin-2 receptor gamma chain as described in: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain" Ohbo K et al. Blood 1996. 87:956-67.
View our Resources for the NSG™ mouse model, including,
These double mutant mice were produced by breeding female NOD.CB17-Prkdcscid/J (Stock No. 001303) mice with male mice bearing the X-linked B6.129S4-Il2rgtm1Wjl/J allele (Stock No. 003174). The resulting male mice heterozygous for the Prkdcscid allele and hemizygous for the Il2rgtm1Wjl allele were crossed to female NOD.CB17-Prkdcscid/J (Stock No. 001303) mice for eight generations. Heterozygotes were interbred to produce mice homozygous for the Prkdcscid allele and homozygous (females) or hemizygous (males) for the Il2rgtm1Wjl allele.
|Allele Name||severe combined immunodeficiency|
|Gene Symbol and Name||Prkdc, protein kinase, DNA activated, catalytic polypeptide|
|Site of Expression||T and B lymphocytes.|
|Strain of Origin||C.BKa-Ighb/Icr|
|Molecular Note||A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).|
|Allele Name||targeted mutation 1, Warren J Leonard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||[KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull|
|Gene Symbol and Name||Il2rg, interleukin 2 receptor, gamma chain|
|Site of Expression||Primarily lymphoid cells.|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.|
|Mutations Made By|| |
Dr. Warren Leonard, NHLBI, NIH
When maintaining a live colony, females that are homozygous for both the Prkdcscid and Il2rgtm1Wjl alleles are bred to males that are homozygous for Prkdcscid and hemizygous for Il2rgtm1Wjl (Il2rg is an X-linked gene). SPF (specific pathogen-free) conditions are recommended.
Related Genotype: A/A Tyrc/Tyrc
When using the NSG mouse strain in a publication, please cite the originating article(s) and include JAX stock #005557 in your Materials and Methods section.
|Volume pricing is not available for Domestic Commercial & For-Profit.|
|Volume pricing is not available for Domestic Not-For-Profit & Academic.|
|Volume pricing is not available for International Commercial & For-Profit.|
|Volume pricing is not available for International Not-For-Profit & Academic.|
Volume Pricing Program
Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached
Sexes: Sexes of the same strain may be combined to reach minimum quantity levels to receive the volume pricing
Shipment: All shipping destinations qualify
This strain is available from some international Charles River (CR) breeding facilities in Japan and/or Europe. For more information, see the Worldwide Distributor List for JAX® Mice.
|Timed Pregnant||Specific Gestational Day (E11-E15)||$1059.65|
|Timed Pregnant||Range of Gestational Days (E11-E15)||$1059.65|
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|Timed Pregnant||Range of Gestational Days (E11-E15)||$530.40|
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|Timed Pregnant||Range of Gestational Days (E11-E15)||$689.52|
|Timed Pregnant||Specific Gestational Day (E11-E15)||$1345.79|
|Timed Pregnant||Range of Gestational Days (E11-E15)||$1345.79|
|Timed Pregnant||Specific Gestational Day (E11-E15)||$1487.27|