These NRG mice are NOD-congenic mice harboring the Rag1null mutation (Rag1KO or Rag1tm1Mom) on chromosome 2 and the IL2rγnull mutation (IL2RγcKO or Il2rgtm1Wjl) on the X chromosome. These NRG mutant mice are also called NOD-Rag1null IL2rγnull double mutant mice or NOD.Rag1KO.IL2RγcKO mice. These NRG mice may be useful for cell or tissue transplantation studies, particularly as a model for human lymphohematopoietic cell engraftment studies that require a radioresistant host.
Dr. Leonard D. Shultz, The Jackson LaboratoryRead More +
These NRG mice are NOD-congenic mice harboring the Rag1null mutation (Rag1KO or Rag1tm1Mom) on chromosome 2 and the IL2rγnull mutation (IL2RγcKO or Il2rgtm1Wjl) on the X chromosome. These NRG mutant mice are also called NOD-Rag1null IL2rγnull double mutant mice or NOD.Rag1KO.IL2RγcKO mice. Females homozygous for both mutations (and males homozygous for Rag1null; hemizygous for the X-linked IL2rγnull mutation) are viable and fertile. When compared to NOD-scid IL2rgnull mice(NSG mice; Stock No. 005557), these NRG mice tolerate much higher levels of irradiation conditioning. Additionally, NRG mice support higher levels of both human cord blood stem cell engraftment following irradiation-conditioning (leading to multi-lineage hematopoietic cell populations and a complete repertoire of human immune cells, including human T cells) and human peripheral blood mononuclear cells engraftment in unconditioned adult mice with respect to NOD-Rag1null (Stock No. 003729) or NOD-scid (Stock No. 001303) mice. These NRG mice may be useful for cell or tissue transplantation studies, particularly as a model for human lymphohematopoietic cell engraftment studies that require a radioresistant host.
View Flow Cytometry Characterization Data for Immunodeficient JAX Strains
These NRG mice are NOD-congenic mice harboring the Rag1null mutation (Rag1KO or Rag1tm1Mom) on chromosome 2 and the IL2rγnull mutation (IL2RγcKO or Il2rgtm1Wjl) on the X chromosome. These NRG mutant mice (also called NOD-Rag1null IL2rγnull double mutant mice or NOD.Rag1KO.IL2RγcKO mice) were produced by breeding NOD-Rag1null (Stock No. 003729) mice with NOD-scid IL2rgnull (Stock No. 005557)mice. Offspring were intercrossed and bred to be homozygous for the Rag1null mutation, homozygous (for females; or hemizygous for males) for the X-linked IL2rγnull mutation, and wild-type for the scid mutation. The donating investigator reports that the genetic background is equivalent to approximately N9 prior to arrival at The Jackson Laboratory Repository.
|Allele Name||targeted mutation 1, Warren J Leonard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD132-; IL2Rgammanull; Il2rgtm1Wjll; [KO]gammac; gammac-; gc-|
|Gene Symbol and Name||Il2rg, interleukin 2 receptor, gamma chain|
|Gene Synonym(s)||Ab2-183; CD132; CIDX; Cd132; IL-2RG; IMD4; P64; SCIDX; SCIDX1; [g]c; common cytokine receptor gamma chain; common gamma chain; gamma C receptor; gamma(c); gc|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.|
|Mutations Made By|| |
Dr. Warren Leonard, NHLBI, NIH
|Allele Name||targeted mutation 1, Peter Mombaerts|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-; Rag1tm1Mom|
|Gene Symbol and Name||Rag1, recombination activating gene 1|
|Gene Synonym(s)||RAG-1; RNF74; Rag-1; Rag-1|
|Site of Expression||expression is seen in bone marrow derived cell lines.|
|Strain of Origin||129S7/SvEvBrd-Hprt<+>|
|Molecular Note||A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.|
|Mutations Made By|| |
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
When maintaining a live colony, females homozygous for both the Rag1null and IL2rγnull mutations may be bred with males homozygous for the Rag1null mutation and hemizygous for the X-linked IL2rγnull mutation. The donating investigator suggests that NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729), NOD/ShiLtJ (Stock No. 001976), or NOD/ShiLtSz may be appropriate controls.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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