Overview

Also Known As:NSG B2m, NOD-scid Il2rg<null> B2m<null>, NOD-scid gamma B2m, NSG-B2m KO

These NOD scid Il2ry^null B2m^null triple mutant mice combine the features of the severe combined immune deficiency mutation (scid), IL2 receptor gamma chain deficiency, and the MHC class I molecule (beta-2 microglobulin) deficiency and are relatively resistant to graft versus host disease (GVHD). They provide a model useful to study in vivo mechanisms of xenogeneic GVHD and to rapidly assess therapeutic agents.

The serum half-life of human IgG is greatly reduced in B2m mutant strains due to defective FcRn function (Junghans and Anderson, 1996, Praetor and Hunziker, 2002).

Donating Investigator

Dr. Leonard D. Shultz, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
005557 NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

B2m^tm1Unc

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	B2m	beta-2 microglobulin

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

View Genetics

Research Applications

Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Research Tools
Metabolism Research
Cancer Research
Hematological Research

View All Research Applications

Base Price

Starting at:

$225.42 Domestic price for male 4-week

View Price List

Details

Detailed Description

The NOD.Cg-Prkdc^scid B2m^tm1Unc Il2rg^tm1Wjl/SzJ mice, commonly known as NOD-scid Il2ry^null B2m ^null (NSG-B2m), do not express the Prkdc gene, the X-linked Il2rg gene nor the B2m gene. Triple mutant mice are viable, normal in size and do not display any gross physical or behavioral abnormalities, but can be poor breeders. Mutant mice combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid), IL2 receptor gamma chain deficiency and the MHC class I molecule, beta-2 microglobulin, deficiency and are relatively resistant to graft versus host disease (GVHD). The mean survival time (MST) of NOD-scid Il2ry^null B2m^null irradiated with 2 Gy and injected with 5 x 10⁶ human peripheral blood mononuclear cells (PBMC) is 44 days compared to the MST of 21 days in the NOD-scid Il2ry^null treated similarly. The blood of GVHD affected NOD-scid Il2ry^null B2m^null animals exhibited decreased human CD45⁺ cell engraftment and significantly higher CD4:Cd8 ratios in spleen and bone marrow when compared with GVHD affected NOD-scid Il2ry^null. This model may be useful to study in vivo mechanisms of xenogeneic graft versus host disease and to rapidly assess therapeutic agents.

Please note that this strain carries the true null interleukin-2 receptor gamma chain mutation and should not be confused with other strains that express a truncated interleukin-2 receptor gamma chain as described in: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain" Ohbo K, et al. Blood 1996. 87:956-67.

This strain ships with a JAXTag^TM affixed. Learn more about JAXTag^TM.

Development

Triple mutant mice were produced by breeding NOD/LtSz-Prkdc^scid B2m^tm1Unc/Sz (Stock No. 003449) mice with NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/Sz (Stock No. 5557) mice. The resulting mice were interbred to produce mice homozygous for the Prkdc^scid and B2m^tm1Unc alleles and homozygous (females) or hemizygous (males) for the X-linked Il2rg^tm1Wjl allele. The T1DR received this stock in 2009.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

King MA; Covassin L; Brehm MA; Racki W; Pearson T; Leif J; Laning J; Fodor W; Foreman O; Burzenski L; Chase TH; Gott B; Rossini AA; Bortell R; Shultz LD; Greiner DL. 2009. Human peripheral blood leucocyte non-obese diabetic-severe combined immunodeficiency interleukin-2 receptor gamma chain gene mouse model of xenogeneic graft-versus-host-like disease and the role of host major histocompatibility complex. Clin Exp Immunol 157(1):104-18PubMed: 19659776 MGI: J:151390

View All References

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

B2m^tm1Unc

Allele Symbol: B2m^tm1Unc

Allele Name	targeted mutation 1, University of North Carolina
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	b2m^null; beta2M-; beta2m⁰; beta2m^null; beta2m^o; beta2m^tm1Unc; beta2MKO; beta2-m-KO; I⁰; MHC-I^-
Gene Symbol and Name	B2m, beta-2 microglobulin
Gene Synonym(s)
Strain of Origin	129P2/OlaHsd
Chromosome	2
Molecular Note	Insertion of a neomycin-resistance gene into the second exon.
Mutations Made By	Dr. Oliver Smithies, University of North Carolina at Chapel Hill

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host

Genotype: B2m^tm1Unc related

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - MHC class I deficiency
Metabolism Research
- Hemochromatosis
  - iron metabolism defects
Hematological Research
- Anemia, Iron Deficiency and Transport Defects
  - hemochromatosis
Internal/Organ Research
- Liver Defects
  - hemochromatosis
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - MHC class I deficiency

Genotype: Il2rg^tm1Wjl related

Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B, T, and NK cell deficiency
- Toxicology Research
  - B and T cell deficiency, xenograft transplant host
Immunology, Inflammation and Autoimmunity Research
- Graft vs. Host Disease

Mammalian Phenotype Terms by Genotype

References

King MA; Covassin L; Brehm MA; Racki W; Pearson T; Leif J; Laning J; Fodor W; Foreman O; Burzenski L; Chase TH; Gott B; Rossini AA; Bortell R; Shultz LD; Greiner DL. 2009. Human peripheral blood leucocyte non-obese diabetic-severe combined immunodeficiency interleukin-2 receptor gamma chain gene mouse model of xenogeneic graft-versus-host-like disease and the role of host major histocompatibility complex. Clin Exp Immunol 157(1):104-18PubMed: 19659776 MGI: J:151390

Additional - B2m^tm1Unc related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- Standard PCR:B2m
- Standard PCR:Il2rg
- Standard PCR:B2m
- Probe:B2m Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred by crossing female mice that are heterozygous for the B2m^tm1Unc allele and homozygous for the Prkdc^scid allele, and homozygous for the X-linked Il2rg^tm1Wjl allele to male mice that are homozygous for the B2m^tm1Unc and Prkdc^scid alleles and hemizygous for the X-linked Il2rg^tm1Wjl allele.
- Additional Breeding and Husbandry Support
Mating System
- See "Breeding Considerations"
Appearance
- albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NSG B2m mouse strain in a publication, please cite the originating article(s) and include JAX stock #010636 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

RB05 (Maximum)

AX27 (Maximum)

Pricing & Availability

Readily Available

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Please inquire

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NSG B2m, NOD-scid Il2rg<null> B2m<null>, NOD-scid gamma B2m, NSG-B2m KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Prkdcscid

Allele Symbol: Prkdcscid

B2mtm1Unc

Allele Symbol: B2mtm1Unc

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Genotype: B2mtm1Unc related

Genotype: Il2rgtm1Wjl related

Mammalian Phenotype Terms by Genotype

References

Additional - B2mtm1Unc related

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Genotyping Protocols

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

B2m^tm1Unc

Allele Symbol: B2m^tm1Unc

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Genotype: Prkdc^scid related

Genotype: B2m^tm1Unc related

Genotype: Il2rg^tm1Wjl related

Additional - B2m^tm1Unc related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related