What We Do
Genome Technologies (GT) is dedicated to propelling research forward through the expert application of cutting-edge next-generation sequencing technologies in support of diverse research programs across JAX. Available platforms include multiple Illumina short-read instruments, Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) long-read systems, and Sanger sequencing. GT is fully equipped to handle various biological sample types and library preparation strategies for genome-wide genetic, epigenetic, and gene expression profiling, as well as targeted sequencing. By partnering with investigators, we empower them to embrace innovative approaches to unravel the complexities of biological systems. Standardized data analysis is provided for certain workflows.
Services and Technologies Offered
- Next-generation sequencing on multiple platforms (e.g., Illumina, PacBio, ONT)
- Nucleic acid extraction and library preparation for DNA and RNA analyses
- Whole-genome sequencing
- RNA-seq including long-read approaches (Kinnex, IsoSeq, ONT)
- Epigenomic profiling techniques of chromatin (e.g., ChIP-seq, ATAC-seq, Hi-C, Fiber-seq)
- DNA methylation analysis (e.g., bisulfite sequencing, enzymatic-conversion, and conversion-free approaches)
- Targeted short- and long-read sequencing (amplicons, capture panels, amplification-free methods)
- Direct sequencing of RNAs
- Sequencing of full-length plasmids, BACs, and long-range PCR sequencing products
- Sanger sequencing
- Support for deployment of new sequencing-centric strategies based on user requests
- Bioinformatics pipelines and software for data analysis
- Temporary data storage and management solutions for large-scale genomic datasets and long-term archival storage
- GT provides sequencing for the Single Cell Biology Laboratory’s extensive suite of single-cell profiling offerings
