CRISPR/Cas9 Service

As an early adopter of the CRISPR/CAS9 technology, The Jackson Laboratory has been exploring the capabilities and limitations of the technology for generating mouse models. We guide you from concept to phenotypic characterization, allowing you and your staff to focus on the research instead of breeding mice.

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Mouse Models Made Simple

We have the experience to deliver a range of mutational approaches on diverse strain backgrounds to make the model you need for your research.

Demonstrated capability. From simple indel KOs to conditional KOs and reporter insertions, we’ve created a wide range of models using CRISPR/Cas9 technology.

No need to backcross your new model to make it congenic. We can generate the model you need on the genetic background you want. We’ve generated models in a variety of strain backgrounds including BALB/cJ, C57BL/6J, C57BL/6NJ, FVB/NJ, NOD/ShiLtJ, and NSG™, and are adding new strains regularly.

Avoid unplanned costs and delays. JAX mice are accepted by most facilities without the need for quarantine or rederivation.

Project support from initial design to getting your mice. Our mouse geneticists will work with you to design a model to meet your needs and once a project begins, we will be there to keep you informed and answer any questions you may have along the way.

Stay focused on your goals. We can further support your research by generating breeding pairs, cohorts of mice, cryopreservation of your new strain and phenotypic characterization. Let your staff focus on research instead of the design, creation, and breeding of Genetically Modified Mice. 

CRISPR/Cas9 Mediated Model Generation Process

When you partner with us to build your model, we keep it simple for you so you can stay focused on your research. The process includes 5 easy steps. We will:

  1. Discuss your goals to develop a project design that will provide you with a detailed strategy of how we will generate your model.
  2. Provide you with regular updates on your project.
  3. Develop a founder data packet and share your results.
  4. Develop an N1 data packet and share your results.
  5. Deliver your N1 mice to you, or provide you with options to quickly expand the colony and cryopreserve your new strain.
You will not be charged for the service if JAX is unable to generate the model as agreed upon in the Project Design Packet, with the exception of unforeseen issues resulting from the specific genetic modification you request.  In some cases, the desired genetic modification may result in a decrease in viability or fertility, or increased morbidity, resulting in the inability to generate the model.  In addition, the genetic modification may not result in the predicted expression pattern or phenotype even if the modification is performed correctly.  Please note that our delivery timelines are estimates only.

Timeline for Creating N1 Mouse Models

 Model Type Timeline (Weeks)
 Indel-mediated KO  26-30
 Deletion-mediated KO  27-31
 Small KI
 Stop codon insertion
 Point mutation
 Tag insertion
 27-31
 Large KI (including ROSA26 locus)
 Reporter insertion
 Conditional KO
 LoxP site insertion
 33-37

Additional Services

You can save time and effort when you opt for additional services to support your specific project goals.

Getting Started

Let us build the mouse model you need. 

To learn more about our CRISPR/Cas9 Mouse Model Generation Services or to set up a project, please call 1-800-422-6423 or email micetech@jax.org.

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  • Case Study: Generation of a SNP Allelic Series to Study FALS

    JAX MGS utilized a short oligo-mediated knockin approach to generate a small mouse model panel in which each model carries a mouse allele that has been modified to carry a different, specified amino acid at key orthologous positions identified in the FALS patient analysis.

  • Webinar: CRISPR/Cas9 - Moving from Founder Mice to Phenotyping

    CRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relatively simple, many researchers struggle with understanding the complexities associated with the diverse alleles that can be generated, how to deal with mosaicism, and how to move from a single founder mouse to larger cohorts for phenotype characterization.