Manuals and Guides

  • Case Study: Creation of a Cre-dependent Fxn Knockout Allele for Friedreich’s Ataxia

    To support a collaboration between Dr. Cat Lutz of The Jackson Laboratory and the Friedrich’s Ataxia Research Alliance, our Model Generation Services (MGS) team undertook a project to create a conditional knockout allele of the Frataxin (Fxn) gene using the Cre-loxP Recombination System.

  • Guide: Aged C57BL/6J Mice for Research Studies

    Mice are used extensively in aging research due to their genetic and physiological similarity to humans. Aging studies are—by necessity—lengthy, resource intensive, and require advance planning. The Jackson Laboratory now offers study-ready cohorts of male and female C57BL/6J mice up to 78 weeks of age. This article is intended to support investigators who may be new users of this resource and more experienced researchers who may be seeking a quick and useful summary. We will review special considerations for maximizing the translational value of data collected from aged mice and highlight resources for identifying and implementing best practices.

  • Guide: Generating Mouse Models with CRISPR/Cas9

    Genome editing with CRISPR/Cas9 enables the generation of new mouse models with unprecedented speed and simplicity. The Jackson Laboratory was an early adopter of CRISPR/Cas9 technology for mouse model development, and in this brief guide we describe the technology and lessons learned to generate knockouts and knockins in more than a dozen different genetic backgrounds.

  • Guide: Humanized Mice for Immuno-Oncology

    NSG™ mice are a proven host for engraftment of human tumors or establishment of human immunity following hematopoietic stem cell transplantation. This guide explains why the interactions between human immune cells and tumors are paramount when devising treatment strategies that prevent tumor evasion of immune cells and improve cytotoxic responses.

  • Metabolic Disease Models Poster

    This poster compares the benefits and considerations for the top 10 metabolic disease mouse models from JAX.

  • Guide: Improved In Vitro Fertilization via Zona Thinning

    Mouse In Vitro Fertilization (IVF) can be used to rapidly expand mouse lines from a few males that carry the desired genotype or to maintain strains with poor breeding efficiency. This guide describes a detailed method using acidified Tyrode solution to open the zona pellucida and increase the fertilization capacity of frozen mouse sperm.

  • Guide: Working with Amyotrophic Lateral Sclerosis (ALS) Mouse Models

    This guide summarizes the guidelines for preclinical testing and colony management of ALS mice discusses considerations for preclinical studies using SOD1 mice, colony management, QPCR protocol for determining copy number, and more.

  • MHC H2 Haplotypes and Histocompatibility Haplotypes and Loci

    Download a listing of MHC H2 Haplotypes and Histocompatibility Haplotypes and Loci for JAX® Mice.

  • Booklet: Mouse Models

    The Jackson Laboratory is the global leader in mammalian genetics modeling and education, and has been a driving force in supporting scientific breakthroughs for over 80 years.

  • Booklet: Oncology

    This booklet explains how JAX® Mice, Clinical and Research Services for oncology are essential tools for the in vivo study of hematological malignancies, neoplasia, regenerative medicine, and immune-oncology. Our PDX models and other next-generation cancer modeling platforms allow you to address critical questions in oncology research, such as unexplained drug resistance, drug efficacy, genomic heterogeneity in solid tumor, and the role of the immune system in drug response.

  • Fact Sheet: JAX Mice for Alhzeimer's Disease Research

    This fact sheet compares JAX® Mice for Alzheimer's disease research.

  • Portfolio: PDX Models and Services

    This portfolio discusses why PDX models provide a relevant and translatable animal model to study human cancer biology compared to cell-line xenografts. Our PDX program provides tumor models at a lower passage, more accurately reflecting clinical samples compared to other PDX resources.

  • Onco-Hu Portfolio

    Patient-derived tumor xenografts in humanized NSG™ and NSG™-SGM3 mice

  • Fact Sheet: ActionSeq™

    The JAX ActionSeq™ test is a targeted panel of 212 cancer related genes analyzed using next-generation sequencing. The panel assesses all identified functional variants for clinical relevance, based on associations in the biomedical literature with response or resistance to FDA-approved targeted therapies.

  • Fact Sheet: FusionSeq™

    Every patient is unique. So is every patient’s tumor. This one pager explains how advances in high-throughput molecular diagnostics now allow detection of the genetic alterations that are specific to an individual patient’s tumor. By identifying the oncogenic drivers, therapeutic options can be matched to the tumor’s biology.