About
This hands-on workshop teaches participants how to make and manipulate mouse embryos to generate new models and efficiently manage mouse strains. Hands-On Instruction Includes:
- How to generate mouse embryos via In-vitro fertilization and natural mating with oviduct flushing
- CRISPR reagent delivery for novel mouse model development via electroporation of zygotes
- Surgical and non-surgical (NSET) embryo transfer
- Embryo cryopreservation and recovery (controlled rate and vitrification methods)
- Microcapillary transfer pipetting
- Embryo evaluation
- Vasectomy surgery
- Selecting mice in estrus
- Male and female reproductive anatomy
Didactic instruction includes how to generate new mouse models using CRISPR technology, how to generate pseudopregnant females, cryobiology, reproductive anatomy, aseptic surgical techniques, biomethods as well as IVF (strain selection, superovulation, project planning and trouble shooting). Participants will obtain the information they need to implement these hands-on techniques when they return to their institution.
The Check-in Date for the Highseas lodging option will be Sunday evening, May 15 and the Check-Out Date will be Friday morning, May 20.
Congratulations Bryhan Mendez of Joslin Diabetes Center, recipient of the 2022 Kent Scientific Scholarship award!
Completion of this workshop may be used for continuing educational units for AALAS re-certification.
