Workshop on Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse

Arrival Day

4:00-7:00 pm Registration/Check in - Highseas Lobby
5:30 Reception - Highseas Lobby
6:00 Dinner – Highseas Dining Room

Monday, April 18, 2016

7:00-7:45              Breakfast - Highseas

7:45-8:00    Transportation to GRB 3225

8:00-8:30    Course Overview and Introductions (GRB 3225)
    Robert Taft, Ph.D., The Jackson Laboratory

8:30-9:30    Assisted Reproductive Technologies – ARTs  (GRB 3225)
       Robert Taft, Ph.D., The Jackson Laboratory

9:30-9:45    Break

9:45-10:45    Anesthesia, Analgesia and Aseptic Surgery Methods  (lecture in GRB 3245)    Bonnie Lyons, D.V.M., The Jackson Laboratory

10:45-12:15    Biomethods (Lab, GRB 3245)
    Kristin Cough, B.S., L.V.T., The Jackson Laboratory

12:15-1:15    Lunch (Roscoes)

1:15-2:15    Cryobiology (GRB 3225)
    Robert Taft, Ph.D., The Jackson Laboratory

2:15-2:30    Break

2:30-4:30    Concurrent Electives (Lab, GRB 3245)
•    Microcapillary Transfer Pipetting - Bree Roberts, The Jackson Laboratory
•    Pulling Microcapillary Transfer Pipettes - Jennifer Corrigan, M.S., The Jackson Laboratory
•    Flushing Embryos - Leanne Micelli, The Jackson Laboratory
•    Selecting Mice in Estrus for the Generation of Pseudopregnant Females - Shannon Byers, M.S., M.B.A. , The Jackson Laboratory

4:30-4:45    Clean-up
    
4:45    Transportation to Highseas

6:00    Dinner at Highseas

Tuesday, April 19, 2016

7:00-7:45               Breakfast - Highseas

7:45-8:00    Transportation to Building 64, B115

8:00-9:00    Liquid Nitrogen Safety, Dry Shippers, Biobank (Bld 64, B115)
    Kurt Christiansen or Jennifer Richardson, The Jackson Laboratory

9:00-9:15    Break

9:15-9:45    Male and Female Reproductive Anatomy (lecture in the lab)
    Robert Taft, Ph.D., The Jackson Laboratory


9:45-11:15      Male and Female Anatomy: Demonstration and Practical (Lab, GRB 3245)
    Shannon Byers, M.S., M.B.A. , The Jackson Laboratory

11:15-11:45     Sperm Cryopreservation (lecture in the lab)
    Steven Griswold, B.A., B.S., The Jackson Laboratory

11:45-12:45    Lunch (Roscoes)

12:45-2:15     Sperm Collection and Cryopreservation (Lab, GRB 3245)
    Jane Farley, The Jackson Laboratory
    Myrna Bragg, The Jackson Laboratory
    Jennifer Corrigan, M.S., The Jackson Laboratory

2:15-2:30    Break

2:30-3:30    In-Vitro Fertilization Planning: Strain Selection, Superovulation and
    Project Planning (GRB 3225)
    Shannon Byers, M.S., M.B.A., The Jackson Laboratory

3:30-4:15    In-Vitro Fertilization Preparation: Media, Dishes and More (Lab, GRB 3245)
    Jane Farley and staff, The Jackson Laboratory

4:15-5:00    Computerized Sperm Analysis Using CEROS II (Lab, GRB 3245)
                  Nancy Mutch, Hamilton Thorne, Inc.

5:00-5:15    Clean-up

5:15    Transportation to Highseas

6:00    Dinner at Highseas

Wednesday, April 20, 2016

7:00-7:45               Breakfast - Highseas

7:45-8:00    Transportation to Training Lab, GRB 3245

8:00-10:00    In-Vitro Fertilization Using Fresh and Frozen Sperm (Lab, GRB 3245)
    Jane Farley and staff, The Jackson Laboratory

10:00-10:15     Break

10:15-12:15     Concurrent Electives
•    Vasectomy (Lab, GRB 3245) - Jennifer Corrigan, M.S., The Jackson Laboratory
•    Ovarian Transplant (Lab, GRB 3245) - Bree Roberts, The Jackson Laboratory
•    Ask the Expert-Bring your questions (GRB 3245) - Robert Taft, Jane Farley and Shannon Byers, The Jackson Laboratory (conference room)
•    Hands-on Computerized Sperm Analysis Using CEROS II   - Nancy Mutch, Hamilton Thorne, Inc., Myrna Bragg, The Jackson Laboratory (Lab, GRB 3245)

12:15-1:15    Lunch (Roscoes)

1:15-2:30    In Vitro Fertilization Change-Over (Lab, GRB 3245)
    Jane Farley and staff, The Jackson Laboratory

2:30-2:45    Break

2:45-3:45    IVF Troubleshooting (lecture and discussion, GRB 3225)    
    Shannon Byers, M.S., M.B.A. , The Jackson Laboratory
    Jane Farley, The Jackson Laboratory
    Myrna Bragg, The Jackson Laboratory
3:45-4:45     Implementing ARTS at Your Institution: Quality Control, Data
    Management and Beyond (GRB 3225)
    Robert Taft, Ph.D., The Jackson Laboratory

4:45    Transportation to Highseas

6:00    Dinner at Highseas

April 21, 2016

7:00-7:45               Breakfast - Highseas

7:45-8:00    Transportation to GRB 3245

8:00-9:00    Introduction to Controlled-Rate Freezing (GRB 3245)
    Robert Taft, Ph.D., The Jackson Laboratory

9:00-9:15     Break

9:15-9:45    Count/Evaluate Embryos from IVF (Lab, GRB 3245)
    Jane Farley, The Jackson Laboratory
    Jennifer Corrigan, M.S., The Jackson Laboratory

9:45-11:15     Freeze Embryos in Straws: Controlled Rate Method (Lab, GRB 3245)
    Bree Roberts, The Jackson Laboratory
    Leanne Miceli, The Jackson Laboratory
    Jennifer Corrigan, M.S., The Jackson Laboratory

11:15-11:45     Thawing Embryos Frozen in Straws (Compatible Method) Demonstration
    (Lab, GRB 3245)
                  Bree Roberts, The Jackson Laboratory

11:45-12:45                Lunch

12:45-1:15     Thawing Embryos Frozen in Straws (Lab, GRB 3245)
                   Bree Roberts, The Jackson Laboratory
                   Jennifer Corrigan, M.S., The Jackson Laboratory

1:15-1:45         Surgical Prep and Embryo Transfer Surgery Demonstration (Lab, GRB 3245)
      Bree Roberts, The Jackson Laboratory

1:45 -2:00    Break

2:00-3:30               Embryo Transfer Practice (Lab, GRB 3245)
    Bree Roberts, The Jackson Laboratory
    Leanne Miceli, The Jackson Laboratory
    Jennifer Corrigan, M.S., The Jackson Laboratory


3:30-4:30     Non-Surgical Embryo Transfer (NSET) Demonstration and Practical (Lab, GRB 3245)
    Barbara Stone, Paratechs

4:30-5:00     Discussion and FAQ’s (Lab, GRB 3245)
    Robert Taft, Ph.D., The Jackson Laboratory

5:00    Transportation to Highseas

6:00    Dinner at Highseas