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Notes
This assay will NOT distinguish hemizygous from homozygous transgenic animals.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Expected Results
Transgene = 288 bp
Internal positive control = 324 bp
JAX Protocol
Protocol Primers
| Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
|---|---|---|---|---|---|---|
| 9703 | AGG ACG GCG AGT TCA TCT AC | Transgene Forward | mCherry | |||
| 9704 | TGG TGT AGT CCT CGT TGT GG | Transgene Reverse | mCherry | |||
| oIMR7338 | CTA GGC CAC AGA ATT GAA AGA TCT | Internal Positive Control Forward | ||||
| oIMR7339 | GTA GGT GGA AAT TCT AGC ATC ATC C | Internal Positive Control Reverse |
Strains Using This Protocol
| Stock Number | Strain Name |
|---|---|
| 040785 | B6(Cg)-Notch3tm1.1(NOTCH3)Arbo/J |
| 040890 | B6.FVB-Tg(Hcar2-mRFP)1Soff/J |
| 024046 | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J |
| 040416 | C57BL/6J-Igs2em1(CAG-mCherry,-Cxcr3*,-GFP*)Miji/J |
| 040208 | C57BL/6J-Igs2em1(CAG-RFP*/GFP*)Qsun/J |
| 018542 | FVB-Tg(Kdr-mCherry)1Medi/J |
| 018067 | FVB-Tg(Prism)1849Htz/J |
| 018071 | FVB-Tg(Prism)1861Htz/J |
| 039157 | STOCK Lcn2tm2Bara Tyrc-2J/J |
| 040810 | STOCK Pou2af1em1Dtan/J |
| 014177 | STOCK Tg(Afp-mCherry)1Hadj/J |
| 025693 | STOCK Tg(CAG-mCherry/Pxn)#Mgin/KleyJ |
| 017470 | STOCK Tg(Tnc-mCherry)2Pmay/J |
| 011108 | STOCK Tg(Ttr-RFP)1Hadj/J |
| 14 strains use this protocol | |