STOCK Tg(Afp-mCherry)1Hadj/J

Stock number: 014177
Research areas: Research Tools

Description

Afp::mCherry transgenic mice have the alpha fetoprotein (Afp) promoter/enhancer sequences driving expression of a monomeric red fluorescent protein, mCherry. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements in the developing embryo.

Detailed description

Afp::mCherry transgenic mice have the alpha fetoprotein (Afp) promoter/enhancer sequences driving expression of a monomeric red fluorescent protein, mCherry. Hemizygotes are viable, fertile, and normal in size. Under control of Afp, mCherry labels the visceral endoderm and its derivatives, including the visceral yolk sac and gut endoderm. Expression is also seen in fetal liver and pancreas, and in liver, pancreas, digestive tract and brain of postnatal mice. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements in the developing embryo.

Development

The Afp::mCherry transgene was designed with a monomeric red fluorescent protein, mCherry, driven by alpha fetoprotein (Afp) promoter/enhancer sequences, followed by a rabbit β-globin polyadenylation site. The transgene was microinjected into fertilized C57BL/6 oocytes and founder mice were bred to ICR outbred mice to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(Afp-mCherry)1Hadj
Name: transgene insertion 1, Anna-Katerina Hadjantonakis
MGI accession ID: MGI:4849505
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Afp-mCherry)1Hadj
Marker name: transgene insertion 1, Anna-Katerina Hadjantonakis
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: RFP,Red Fluorescent Protein,coral
Molecular note: The Afp::mCherry transgene was designed with a monomeric red fluorescent protein, mCherry, driven by alpha fetoprotein (Afp) promoter/enhancer sequences, followed by a rabbit beta-globin polyadenylation site. Line 1 was generated. Under control of Afp, mCherry labels the visceral endoderm and its derivatives, including the visceral yolk sac and gut endoderm. Expression is also seen in fetal liver and pancreas, and in liver, pancreas, digestive tract, and brain of postnatal mice.