These transgenic mice carry a BAC encoding a diphtheria toxin receptor-mCherry fusion protein (DTR-mCherry) preceded by a loxP-flanked transcriptional Stop element under the control of the Csf1r promoter. They are part of a two-gene approach to target monocytes and macrophages, but not conventional dendritic cells (cDCs).
Michel C Nussenzweig, The Rockefeller University
Genetic Background | Generation |
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?+pN3F2
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
Cells of the mononuclear phagocytic lineage are essential for both innate and adaptive immunity. This strain is part of a two-gene approach to target monocytes and macrophages, but not conventional dendritic cells (cDCs).
These transgenic mice carry a BAC encoding a diphtheria toxin receptor-mCherry fusion protein (DTR-mCherry) preceded by a loxP-flanked transcriptional Stop element under the control of the Csf1r promoter. Expression of cre recombinase excises the Stop element and allows transcription and translation of DTR-mCherry in cells expressing Csf1r. Animals expressing DTR-mCherry can be treated with diptheria toxin to selectively ablate these cell populations.
Deletion of the floxed Stop element through crosses with Lyz2 (lysozyme 2)-cre mice (see Stock No. 004781) leads to expression of DTR-mCherry in splenic monocytes but not cDCs of compound mutant mice. Ly6ChighCD115+ spleen monocytes uniformly express mCherry, whereas MHCIIhighCD11chigh cDCs do not. Single injections of diptheria toxin in these animals (4 ng/g body weight) result in a complete loss of peripheral blood and bone marrow monocytes after 24 hours. Peripheral blood Ly6Chigh monocytes return to normal levels after 3 days and are present in super-physiological levels in blood on days 4 and 5 before returning to baseline after day 6.
A transgenic BAC vector incorporating a loxP-Stop-loxP-IRES-DTR/mCherry fusion protein construct in intron 21 of the mouse Csf1r gene was introduced to C57BL/6 embryos. This strain was maintained on a C57BL/6 genetic background by the donating laboratory.
Expressed Gene | HBEGF, heparin binding EGF like growth factor, human |
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Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Monocytes and macrophages. Deletion of the floxed Stop element leads to expression of DTR-mCherry in splenic monocytes. |
Allele Name | transgene insertion 1, Michel C Nussenzweig |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Csf1rLsL-DTR; Tg(Csfr1r-DTR/mCherry)1Mnz |
Gene Symbol and Name | Tg(Csf1r-HBEGF/mCherry)1Mnz, transgene insertion 1, Michel C Nussenzweig |
Gene Synonym(s) | |
Promoter | Csf1r, colony stimulating factor 1 receptor, mouse, laboratory |
Expressed Gene | HBEGF, heparin binding EGF like growth factor, human |
Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Monocytes and macrophages. Deletion of the floxed Stop element leads to expression of DTR-mCherry in splenic monocytes. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A transgenic BAC vector incorporating a loxP-Stop-loxP-IRES-HBEGF(DTR)/mCherry fusion protein construct in intron 21. Line 1 was generated. |
Hemizygotes are viable and fertile.
When using the C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024046 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Csf1r-HBEGF/mCherry)1Mnz |
Frozen Mouse Embryo | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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