STOCK Tg(CAG-GFP,-mCherry/Pxn)#Mgin/KleyJ

Stock number: 025693
Research areas: Cell Biology Research, Hematological Research, Research Tools

Description

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers who register interest will be contacted when the strain is again available.

Flag-mCherry-paxillin transgenic mice express widespread GFP and Cre recombinase dependent flag-tagged mCherry/paxillin fusion protein. This strain may be useful in studies of focal adhesions and neutrophil rolling.

This strain is genotyped using primers for mCherry (generic mCherry). At this time we do not have an allele specific assay for the flag-mCherry-paxillin transgene [September 2015].

Detailed description

These transgenic mice express a floxed GFP and a Cre recombinase mediated flag-tagged mCherry/paxillin fusion under the direction of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter. The mCherry was fused to the N-terminus of a mouse paxillin cDNA. Paxillin is a cytoskeletal protein that is involved in focal adhesions, mediation of protein-protein interactions and the ephrin type a receptor 2 /focal adhesion kinase /Paxillin (EphA2/FAK/Paxillin) signaling pathway. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed GFP and floxed neomycin STOP cassette deleted in the cre-expressing tissues allowing expression of the flag-tagged mCherry/paxillin fusion protein at focal adhesions. It is not known if homozygotes are viable.

Development

A transgenic construct containing a loxP flanked GFP cDNA, a loxP flanked neomycin STOP cassette followed by a flag-tagged mCherry/paxillin fusion gene, all under the control of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, was injected into fertilized mouse eggs. The mCherry was fused to the N-terminus of a mouse paxillin cDNA. This founder line was subsequently established on the mixed C57BL/6J and Sv129 /J background. The mice were then backcrossed to a congenic B6.129P2 strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(CAG-GFP,-mCherry/Pxn)#Mgin
Name: transgene insertion, Mark Ginsberg
MGI accession ID: MGI:5661575
Generation method: Transgenic
Attributes: Reporter; Inserted expressed sequence
Synonyms: Flag-mCherry-paxillin
Marker symbol: Tg(CAG-GFP,-mCherry/Pxn)#Mgin
Marker name: transgene insertion, Mark Ginsberg
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: GFP,Green Fluorescent Protein,; RFP,Red Fluorescent Protein,coral; Pxn,paxillin,mouse, laboratory
Site of expression: Expression of the floxed GFP and a Cre recombinase mediated flag-tagged mCherry/paxillin fusion protein is widespread.
Molecular note: A transgenic construct containing a loxP flanked GFP cDNA, a loxP flanked neomycin STOP cassette followed by a flag-tagged mCherry/paxillin fusion gene under the control of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, was injected into fertilized mouse eggs. The mCherry was fused to the N-terminus of a mouse paxillin cDNA.