Flag–mCherry–paxillin transgenic mice express widespread GFP and Cre recombinase dependent flag-tagged mCherry/paxillin fusion protein. This strain may be useful in studies of focal adhesions and neutrophil rolling.
This strain is genotyped using primers for mCherry(generic mCherry). At this time we do not have a allele specific assay.
Klaus Ley, La Jolla Institute for Allergy & Immunology
Mark Ginsberg, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Reporter, Inserted expressed sequence) |
This strain is genotyped using primers for mCherry(generic mCherry). At this time we do not have a allele specific assay.
These transgenic mice express a floxed GFP and a Cre recombinase mediated flag-tagged mCherry/paxillin fusion under the direction of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter. The mCherry was fused to the N-terminus of a mouse paxillin cDNA. Paxillin is a cytoskeletal protein that is involved in focal adhesions, mediation of protein-protein interactions and the ephrin type a receptor 2 /focal adhesion kinase /Paxillin (EphA2/FAK/Paxillin) signaling pathway. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed GFP and floxed neomycin STOP cassette deleted in the cre-expressing tissues allowing expression of the flag-tagged mCherry/paxillin fusion protein at focal adhesions. It is not known if homozygotes are viable.
A transgenic construct containing a loxP flanked GFP cDNA, a loxP flanked neomycin STOP cassette followed by a flag-tagged mCherry/paxillin fusion gene, all under the control of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, was injected into fertilized mouse eggs. The mCherry was fused to the N-terminus of a mouse paxillin cDNA. This founder line was subsequently established on the mixed C57BL/6J and Sv129 /J background. The mice were then backcrossed to a congenic B6.129P2 strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | Pxn, paxillin, mouse, laboratory |
Site of Expression | Expression of the floxed GFP and a Cre recombinase mediated flag-tagged mCherry/paxillin fusion protein is widespread. |
Allele Name | transgene insertion, Mark Ginsberg |
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Allele Type | Transgenic (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Flag-mCherry-paxillin |
Gene Symbol and Name | Tg(CAG-GFP,-mCherry/Pxn)#Mgin, transgene insertion, Mark Ginsberg |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | Pxn, paxillin, mouse, laboratory |
Site of Expression | Expression of the floxed GFP and a Cre recombinase mediated flag-tagged mCherry/paxillin fusion protein is widespread. |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | A transgenic construct containing a loxP flanked GFP cDNA, a loxP flanked neomycin STOP cassette followed by a flag-tagged mCherry/paxillin fusion gene under the control of the CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, was injected into fertilized mouse eggs. The mCherry was fused to the N-terminus of a mouse paxillin cDNA. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). It is not known if homozygotes are viable.
When using the STOCK Tg(CAG-GFP,-mCherry/Pxn)#Mgin/KleyJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #025693 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(CAG-GFP,-mCherry/Pxn)#Mgin |
Frozen Mouse Embryo | STOCK Tg(CAG-GFP -mCherry/Pxn)#Mgin/KleyJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-GFP -mCherry/Pxn)#Mgin/KleyJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-GFP -mCherry/Pxn)#Mgin/KleyJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(CAG-GFP -mCherry/Pxn)#Mgin/KleyJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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