These pLD53.SC2-mCherry express mCherry under the direction of the Tenascin C (Tnc) promoter/enhancer region within a BAC transgene. Expression is observed in a number of tissues during embryonic development.
Peter Maye, University of Connecticut Health Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for the pLD53.SC2-mCherry transgene are viable and fertile with mCherry expression directed by the Tenascin C (Tnc) promoter/enhancer regions within the BAC transgene. TNC is an extracellular matrix glycoprotein expressed in the developing embryo, and during conditions such as wound healing, inflammation and neoplasia. These mice express mCherry protein in growing and developing tissues of embryo and adults. Specifically, at E10.5 mCherry expression is high in pericardium and by E12.5 expression is detected in the developing craniofacial structures and developing limbs. At E17.5 expression is localized within cartilage. During the first 2 weeks of life, expression is restricted to tendons, ligaments, as well as periosteal and articular surfaces of the bone.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP24-79K20) containing the entire mouse Tenascin C (Tnc) gene. This modified construct was microinjected into fertilized CD1 oocytes. Offspring from founder line 2 were bred to CD1 mice, and were subsequently backcrossed to CD1 for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression |
Allele Name | transgene insertion 2, Peter Maye |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | pLD53.SC2-mCherry |
Gene Symbol and Name | Tg(Tnc-mCherry)2Pmay, transgene insertion 2, Peter Maye |
Gene Synonym(s) | |
Promoter | Tnc, tenascin C, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP24-79K20) containing the entire mouse Tenascin C (Tnc) gene. Line 2 was generated. |
Mutations Made By | Peter Maye, University of Connecticut Health Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier), mice from the colony.
When using the STOCK Tg(Tnc-mCherry)2Pmay/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017470 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Tnc-mCherry)2Pmay |
Frozen Mouse Embryo | STOCK Tg(Tnc-mCherry)2Pmay/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Tnc-mCherry)2Pmay/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Tnc-mCherry)2Pmay/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Tnc-mCherry)2Pmay/J | $3373.50 |
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