Overview

Also Known As:Flk1-myr::mCherry

This strain has been discontinued. The Jackson Laboratory quality control efforts have confirmed very low mCherry intensity in this strain. A suitable alternative may be the Flk1-myr::mCherry transgenic line (Stock No. 032145) or Flk1-GFP knock-in/knock-out line (Stock No. 017006). Please search the JAX Mice database for other strains which may be suitable for your studies.

Flk1-myr::mCherry transgenic mice express a myristoylated mCherry fluorescent protein under control of Kdr regulatory regions specifically in endothelial cells and have applications in in vivo studies of blood vessels and vasculature and for breeding multi-fluorescent protein expressing mutant lines.

Donating Investigator

Mary Dickinson, Baylor College of Medicine

Genetic overview

Genetic Background	Generation

Tg(Kdr-mCherry)1Medi

Allele Type
Transgenic (Reporter)

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

In these transgenic mice mCherry fluorescent protein expression is specific to endothelial cells (myristoylation targets the mCherry expression to the cell membrane). Fluorescence is detected in embryonic endothelium and endocardium as early as 8.5 dpc, in ocular vessels and in capillaries of adult animals. Mice that are homozygous for the transgene are viable, fertile and have a higher transgene expression level than hemizygotes, resulting in brighter fluorescence.

Researchers using this strain have notified The Jackson Laboratory that the mCherry intensity in this mouse line is very low, and may not accurately reflect Kdr expression. Jackson Laboratory quality control efforts have confirmed these observations. Stock No. 017006 and Stock No. 032145 may be suitable alternatives.

Development

In this transgenic construct a mCherry fluorescent protein gene fused to a myristoylation
motif and SV40 polyadenylation site sequence was inserted between the mouse Kdr, kinase insert domain protein receptor, regulatory elements (promoter and enhancer). This construct was injected into fertilized FVB mouse eggs. Founder line 1 was subsequently established. The strain was maintained on the FVB background by the Donating Investigator. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Expression Data

Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Transgenic mice express a myristoylated mCherry fluorescent protein in endothelial cells.

Control Suggestions

Noncarrier
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Larina IV; Shen W; Kelly OG; Hadjantonakis AK; Baron MH; Dickinson ME. 2009. A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development. Anat Rec (Hoboken) 292(3):333-41PubMed: 19248165 MGI: J:155859

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Genetics

Tg(Kdr-mCherry)1Medi

Allele Symbol: Tg(Kdr-mCherry)1Medi

Allele Name	transgene insertion 1, Mary E Dickinson
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	Flk1-myr::mCherry
Gene Symbol and Name	Tg(Kdr-mCherry)1Medi, transgene insertion 1, Mary E Dickinson
Gene Synonym(s)
Promoter	Kdr, kinase insert domain protein receptor, mouse, laboratory
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Transgenic mice express a myristoylated mCherry fluorescent protein in endothelial cells.
Strain of Origin	FVB
Chromosome	UN
Molecular Note	An mCherry reporter with an attached myristoylation motif was placed under the control of Kdr regulatory regions. The Kdr promoter was located 5' and the enhancer element was located 3' of the reporter.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Fluorescent Proteins
Neurobiology Research
- Fluorescent protein expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Larina IV; Shen W; Kelly OG; Hadjantonakis AK; Baron MH; Dickinson ME. 2009. A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development. Anat Rec (Hoboken) 292(3):333-41PubMed: 19248165 MGI: J:155859

Additional - Tg(Kdr-mCherry)1Medi related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Generic mCherry
- QPCR:mCherry QPCR alternate 1
- Standard PCR:Generic mCherry
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
- Hemizygote x Noncarrier

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Flk1-myr::mCherry

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Kdr-mCherry)1Medi

Allele Symbol: Tg(Kdr-mCherry)1Medi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(Kdr-mCherry)1Medi related

Genotyping Protocols

Breeding Considerations

Mating System

Terms Of Use

Additional Use Restrictions Apply

Licensing Information