FVB-Tg(Prism)1861Htz/J

Stock number: 018071
Research areas: Neurobiology Research, Research Tools

Description

This bitransgenic mouse model allows distinct fluorescent visualization of neurons and oligodendrocytes and may be useful for noninvasive and/or in vivo studies of the central nervous system and neural circuits, as well as a source for cell culture experiments.

Detailed description

These transgenic mice express two distinct fluorophores in subsets of cells in the brain: a HA tagged YFP under the control of Mobp for yellow fluorescing oligodendrocytes, and mCherry under the control of Snap25 for red fluorescing neurons. Transgene expression, for each transgenic construct, mimics endogenous gene expression patterns. Hemizygotes are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.

Development

Two mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1861 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The 2 transgenic constructs were then co-injected into FVB fertilized mouse eggs. Founder line 1861 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Allele

Symbol: Tg(Prism)1861Htz
Name: transgene insertion 1861, Nathaniel Heintz
MGI accession ID: MGI:5317668
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Prism)1861Htz
Marker name: transgene insertion 1861, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB
Expressed genes: RFP,Red Fluorescent Protein,coral; YFP,Yellow Fluorescent Protein,jellyfish
Site of expression: HA tagged YFP is expressed in oligodendrocytes, and mCherry is expressed in neurons.
Molecular note: Two mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1861 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. Founder line 1861 was subsequently established.