This bitransgenic mouse model allows distinct fluorescent visualization of neurons and oligodendrocytes and may be useful for noninvasive and/or in vivo studies of the central nervous system and neural circuits, as well as a source for cell culture experiments.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express two distinct fluorophores in subsets of cells in the brain: a HA tagged YFP under the control of Mobp for yellow fluorescing oligodendrocytes, and mCherry under the control of Snap25 for red fluorescing neurons. Transgene expression, for each transgenic construct, mimics endogenous gene expression patterns. Hemizygotes are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.
Two mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1861 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The 2 transgenic constructs were then co-injected into FVB fertilized mouse eggs. Founder line 1861 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | HA tagged YFP is expressed in oligodendrocytes, and mCherry is expressed in neurons. |
Allele Name | transgene insertion 1861, Nathaniel Heintz |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Prism JD1861; Snap25::mcherry Mobp::YFP-HA; Tg(Snap25-mCherry,Mobp-YFP)1861Htz |
Gene Symbol and Name | Tg(Prism)1861Htz, transgene insertion 1861, Nathaniel Heintz |
Gene Synonym(s) | |
Promoter | Mobp, myelin-associated oligodendrocytic basic protein, mouse, laboratory |
Promoter | Snap25, synaptosomal-associated protein 25, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | HA tagged YFP is expressed in oligodendrocytes, and mCherry is expressed in neurons. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | Two mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1861 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. Founder line 1861 was subsequently established. |
When maintaining a live colony, hemizygotes may be bred with wildtype (noncarrier) mice from the colony or FVB/NJ inbred mice (Stock No. 001800).
When using the FVB-Tg(Prism)1861Htz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018071 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Prism)1861Htz |
Frozen Mouse Embryo | FVB-Tg(Prism)1861Htz/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Prism)1861Htz/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Prism)1861Htz/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Prism)1861Htz/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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