| Allele Name | transgene insertion 1849, Nathaniel Heintz |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Prism JD1849; Snap25::mcherry, Mobp::Yfp-HA, Aldh1L1::Cerulean-Myc; Tg(Snap25-mcherry,Mobp-YFP,Aldh1l1-Cerulean)1849Htz |
| Gene Symbol and Name | Tg(Prism)1849Htz, transgene insertion 1849, Nathaniel Heintz |
| Gene Synonym(s) | |
| Promoter | Aldh1l1, aldehyde dehydrogenase 1 family, member L1, mouse, laboratory |
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| Promoter | Mobp, myelin-associated oligodendrocytic basic protein, mouse, laboratory |
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| Promoter | Snap25, synaptosomal-associated protein 25, mouse, laboratory |
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| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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| Expressed Gene | RFP, Red Fluorescent Protein, coral |
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| Expressed Gene | myc tag, myc tag, |
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| Expressed Gene | HA tag, HA tag, |
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| Site of Expression | HA tagged YFP is expressed in oligodendrocytes, Myc tagged CFP is expressed in astrocytes, and mCherry is expressed in neurons. |
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| Strain of Origin | FVB |
| Chromosome | UN |
| Molecular Note | Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into asingle locus on chromosome 11. |
