These transgenic mice express three distinct fluorophores in subsets of cells in the brain: HA tagged YFP under the control of Mobp for yellow fluorescing oligodendrocytes, a Myc tagged Cerulean (CFP) under the control of Aldh1l1 for bluegreen fluorescing astrocytes, and mCherry under the control of Snap25 for red fluorescing neurons. Transgenic mice exhibit a head rearing behavior as early as postnatal day 21, and have impaired locomotor abilities and other behavioral abnormalities. Male hemizygotes do not breed due to the behavioral phenotype. Female hemizygotes make poor mothers. Transgene expression, for each transgenic construct, mimics endogenous gene expression patterns. Cerulean fluorescence is dimmer than YFP and mCherry. mCherry expression increases with age of the mouse.
FISH analysis of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. The Donating Investigator has not attempted to make the strain homozygous.

This tri-allelic Prism JD1849 mouse model allows distinct fluorescent visualization of neurons, astrocytes, and oligodendrocytes and may be useful for noninvasive and/or in vivo studies of the central nervous system and neural circuits, as well as a source for cell culture experiments.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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