This tri-allelic Prism JD1849 mouse model allows distinct fluorescent visualization of neurons, astrocytes, and oligodendrocytes and may be useful for noninvasive and/or in vivo studies of the central nervous system and neural circuits, as well as a source for cell culture experiments.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express three distinct fluorophores in subsets of cells in the brain: HA tagged YFP under the control of Mobp for yellow fluorescing oligodendrocytes, a Myc tagged Cerulean (CFP) under the control of Aldh1l1 for bluegreen fluorescing astrocytes, and mCherry under the control of Snap25 for red fluorescing neurons. Transgenic mice exhibit a head rearing behavior as early as postnatal day 21, and have impaired locomotor abilities and other behavioral abnormalities. Male hemizygotes do not breed due to the behavioral phenotype. Female hemizygotes make poor mothers. Transgene expression, for each transgenic construct, mimics endogenous gene expression patterns. Cerulean fluorescence is dimmer than YFP and mCherry. mCherry expression increases with age of the mouse.
FISH analysis of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. The Donating Investigator has not attempted to make the strain homozygous.
Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. The 3 transgenic constructs were then co-injected into FVB fertilized mouse eggs. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | myc tag, myc tag, |
Expressed Gene | HA tag, HA tag, |
Site of Expression | HA tagged YFP is expressed in oligodendrocytes, Myc tagged CFP is expressed in astrocytes, and mCherry is expressed in neurons. |
Allele Name | transgene insertion 1849, Nathaniel Heintz |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Prism JD1849; Snap25::mcherry, Mobp::Yfp-HA, Aldh1L1::Cerulean-Myc; Tg(Snap25-mcherry,Mobp-YFP,Aldh1l1-Cerulean)1849Htz |
Gene Symbol and Name | Tg(Prism)1849Htz, transgene insertion 1849, Nathaniel Heintz |
Gene Synonym(s) | |
Promoter | Aldh1l1, aldehyde dehydrogenase 1 family, member L1, mouse, laboratory |
Promoter | Mobp, myelin-associated oligodendrocytic basic protein, mouse, laboratory |
Promoter | Snap25, synaptosomal-associated protein 25, mouse, laboratory |
Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | myc tag, myc tag, |
Expressed Gene | HA tag, HA tag, |
Site of Expression | HA tagged YFP is expressed in oligodendrocytes, Myc tagged CFP is expressed in astrocytes, and mCherry is expressed in neurons. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into asingle locus on chromosome 11. |
When maintaining a live colony, female hemizygotes may be bred with wildtype (noncarrier) mice from the colony or FVB/NJ inbred mice (Stock No. 001800). Foster mothers are recommended as female hemizygotes do not make good mothers. Male hemizygotes do not breed due to a behavioral phenotype.
When using the FVB-Tg(Prism)1849Htz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018067 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Prism)1849Htz |
Frozen Mouse Embryo | FVB-Tg(Prism)1849Htz/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Prism)1849Htz/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Prism)1849Htz/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Prism)1849Htz/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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