Graft versus Host Disease (GvHD) Efficacy Studies

With a foundation of JAX’s unrivaled Hu-PBMC-NSG™ mice, our GvHD in-vivo assay gives you access to a vast selection of precharacterized PBMC donor profiles before you even get started. This allows you to better predict survival patterns and study timelines to support your therapeutic development goals.

Features and Benefits OF GvHD STUDIES AT JAX

When working with JAX you are guaranteed:

  • Project support from Ph.D.-level study directors, from initial study design to report analysis
  • Extensive experience conducting large-scale GvHD studies for commercial clients
  • Outcomes compared to a clinically-approved immunosuppressive drug (e.g.,Abatacept)
  • Industry leading Hu-PBMC-NSG™ mice.

Pre-Clinical Screening

The progression and severity of GvHD or xenogeneic T cell activation in Hu-PBMC-NSG™ mice is donor specific, so you can choose the ideal donors for your study needs.

Efficacy testing of novel therapeutics allows you to test immune suppression or immune activation across multiple genetically diverse donors.

Predictability of Donor Response & Easy Donor Selection

Prior knowledge of GvHD response of a donor helps in appropriate donor selection and dosing scheme to align within a drug response window. The collection of human PBMC donors available have been characterized for GvHD response by survival and human cell engraftment in the NSG platform.

Reproducibility and Flexibility to Perform Multiple Studies

GvHD response of pre-characterized PBMC donor is reproducible in repeat studies. A single donor can be used to create larger cohorts of Hu-PBMC-NSG™.

Multiple screening studies can be executed using a preferred PBMC donor to select desirable candidate molecules.

Sufficient cells from a single donor are available to perform longitudinal studies.

Quick Turnaround Time

Average time to completion of study is 35 days.

GvHD Study Design

Standard:

  • Standard study arms (3-4) with vehicle, test article and control treatment
  • Prophylactic or therapeutic treatment
  • Midpoint and terminal blood samples for analysis
  • Disease Activity Index measurements
  • Survival curves
  • Study Summary Report

Optional Add-ons:

  • Immunophenotyping of blood and other tissues such as spleen
  • Cytokines analysis in peripheral blood
  • Tissue collection and histopathology
  • Additional Treatment Arms
  • Alternative benchmarking agent available

The INDUSTRY-LEADing NSG™ Platform

Humanized-PBMC-NSG™ mice are widley used as models of human-to-mouse xenogeneic GvHD, as well as studying immune function in vivo. Peripheral blood mononuclear cells (PBMCs) engraft with high efficiency into NSG™ mice due to the lack of mature murine lymphocytes and natural killer cells. The high degree of immunodeficiency allows engraftment of larger cohorts from a single donor, creating robust and reproducible study platforms.

Learn More about NSG Mice
Learn More about Immune Humanized Mice

BENEFITS OF HuPBMC NSG MICE FOR GvHD

  • They are more representative of the human condition as human immune cells are the target of therapeutic drugs
  • T cell response is robust and reproducible
  • GvHD onset can be manipulated using variable cell dose with or without irradiation
  • Quick turnaround time

View this study example

See an example of study information provided when conducting a GvHD study with the Jackson Laboratory. This example shows bispecific antibodies in comparison with the immunosuppressant drug, Abatacept.

GvHD Efficacy Study Fact Sheet