Humanized CD34+ mice are supreme in vivo models to study immunology, infectious diseases, and more. For example, NSG™engrafted models offer the longest research span, over 12 months with a functional human immune system displaying T-cell dependent inflammatory responses with no donor cell immune reactivity towards the host.
Hu-CD34+ mice produced by injecting CD34+ hematopoietic stem cells (hu-CD34) demonstrate robust multi-lineage engraftment of human immune cell populations including very good T cell maturation and function for long-term studies. JAX has been the pioneer of developing cutting-edge immunodeficient strains such as the NSG™ models and its variants to enable powerful research.
Key features:
- Human CD34+ cells stably engraft the bone marrow.
- Engraftment is stable for over one year without graft-versus-host disease.
- CD4+ and CD8+ T cells are present in circulation and other tissues (Shultz et al. 2005).
- With the hu-IL15, you can test the efficacy of NK-cell mediated therapeutics, including ADCC
- Hu-CD34 NSG models are ideally suited for testing therapeutics with antigen-independent T cell MOA.
- CD34+ hu-NSG-SGM3 are best for therapeutics with T cell and myeloid cell dependent MOA, including those that target AML
Humanization Process
Female mice are injected with human hematopoietic stem cells (hu-CD34+). Engraftment of mature human white blood cells (hCD45+) is confirmed approximately 2 months after injection.
Applications
Hu-CD34-NSG™ and hu-CD34-SGM3 are ideal for long-term in vivo studies in the areas of immunology, immuno-oncology, infectious diseases and more -since their lifespan after CD34+ engraftment is over 12 months.
For more information on using NSG mice and their variants, click here.
Validation
Successful humanization of each mouse is quantified from mouse peripheral blood via flow cytometry using anti- hu-CD45+ and anti-murine CD45+, approximately 2 months post-engraftment, when mature T-cells develop (Ishikawa et al. 2005). All mice ship with >25% hu-CD45+ cells in the peripheral blood.
Availability
Study-ready cohorts of inventoried humanized CD34+ mice are readily available for shipment to your institution or for enrollment in customized drug efficacy studies executed by our experienced Ph.D. level scientists from JAX In Vivo Services. The models readily available include: NSG, NSG-SGM3, NSG-IL15; custom models are available on-demand (for a list of NSG variants, click here).
Licensing Requirements
Companies and for-profit entities require a no-fee license prior to shipping. Many companies already have a license that includes engrafted NSG™ mice. To confirm that your company is covered or for new license inquiries, please contact: techtran@jax.org
Researchers at academic institutions are approved to order engrafted NSG mice.
