CD34+ cell humanized mice feature human hematopoietic stem cells engrafted into mice without a functional immune system. JAX NSG® CD34+ humanized models develop stable, multi-lineage human immune systems. Mature immune cell abundance varies by genetic background (see table below). These models support longer studies and demonstrate diverse mature immune cell populations. They are ideal for evaluating mechanism of action and antibody-dependent cellular cytotoxicity.
Choose Jackson Laboratory immune humanized mice for:
Immuno-ablated female NSG mice are injected with human hematopoietic stem cells (hu-CD34+). Engraftment of mature human white blood cells (hCD45+) is confirmed approximately 2 months after injection.
Available Off-the-Shelf
Study-ready cohorts are prepared regularly and delivered quickly upon order.
Customization
CD34+ HSC engraftment of other NSG and NRG variants are also available.
Leaders in Humanized Mice
Accelerate your breakthroughs with the most reliable source of biologically relevant models.
Expression of distinct human genes support different mature cell populations. Use the table below to choose the best model for your studies.
| Strain Name | Human Gene Expression | Benefits |
|---|---|---|
| CD34+ Hu-NSG™ Stock No. 705557 Naïve Strain Data Sheet | N/A |
|
| CD34+ Hu-NSG-SGM3 Stock No. 701362 Naïve Strain Data Sheet | KITL (SCF), IL3, GM-CSF |
|
| CD34+ Hu-NSG-IL15 Stock No. 703089 Naïve Strain Data Sheet | IL15 |
|
| CD34+ Hu-NSG-SGM3-IL15 Stock No. 703321 Hu-NSG-SGM3-IL15 Data Naïve Strain Data Sheet | KITL (SCF), IL3, GM-CSF, IL15 |
|
| CD34+ Hu-NSG-FLT3-IL15 Stock No. 703722 Hu-NSG-FLT3-IL15 Data Naïve Strain Data Sheet | FLT3 Ligand, IL15 |
|
Successful humanization of each mouse is quantified from mouse peripheral blood via flow cytometry using anti- hu-CD45+ and anti-murine CD45+ (Ishikawa et al. 2005). All mice ship with >25% hu-CD45+ cells in the peripheral blood.
Choose from our standard off-the-shelf models or select models engrafted with particular HLA Type, demonstrating requested engraftment profiles, or other specific made-to-order engraftments.
Companies and for-profit entities require a no-fee license prior to shipping, learn more about the JAX Leap Licensing program. Many companies already have a license that includes engrafted NSG™ mice. To confirm that your company is covered or for new license inquiries, please contact: [email protected]
Researchers at academic institutions are approved to order engrafted NSG mice.
Generated by engraftment of human CD34+ hematopoietic stem cells (HSCs) into our NSG and NSG variants, this platform enables >12-months' functional human immune system analysis, with reduced donor cell immune reactivity towards the host. Optimize your therapeutic development based on the best insights for immune-cell development, cell-cell interactions, and disease-relevant preclinical data with humanized CD34+ models!
CD34 Engrafted SGM3-IL15 (703321)
Tumor infiltration lymphocyte profiles in response to control, single administration, and dual administration demonstrates effective infiltration of relevant cells and reduced CD55 tumor evasion marker under dual administration.
Find out how JAX's enhanced innate immune models enable exploration of cell therapies, antibody therapies, and in vivo immune engineering with:
