Strain Background and Genetic Drift

Contents

A. Strain Background and Genetic Drift FAQ

 

1. What is the genetic background of this strain?

2. What is the right control for this mouse?

3. I’m not sure about the purity of the background of my mouse strain. What can I do?

4. What is the difference between inbred and outbred mice? Inbred and outbred nude mice?

5. What is genetic drift?

6. How do I detect genetic drift?

7. How do I prevent genetic drift?

8. What is the difference between C57BL/6J and C57BL/6N? Or BALB/c and BALB/cByJ? What are substrains?

9. Do I really need to backcross my mutant to the inbred line to “refresh” it?

 

B. Strain Background and Genetic Drift FAQ -Related Articles and Resources

 

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A. Strain Background and Genetic Drift FAQ

1. What is the genetic background of this strain?

Please refer to the “Detail section of the strain datasheet. The way the strain was made will be in the Development section. The background information can also sometimes be clarified by viewing “Control Suggestions.” 

Additionally, the nomenclature may offer some clues:

Click here for more information on nomenclature.

Click here for a tutorial on nomenclature.

2. What is the right control for this mouse?

See Considerations for Choosing Controls and Controls for Genetically Engineered Mice for this information.

3. I’m not sure about the purity of the background of my mouse strain. What can I do?

A Genome Scan can help determine strain background..

4. What is the difference between inbred and outbred mice? Inbred and outbred nude mice?

An inbred strain is one that:

  • is produced using at least 20 consecutive generations of sister x brother or parent x offspring matings, or;
  • is traceable to a single ancestral pair in the 20th or subsequent generation.

An outbred mouse stock is a closed population (for at least four generations) of genetically variable animals that is bred to maintain maximum heterozygosity.

An inbred mouse has more genetic uniformity than an outbred mouse.

5. What is genetic drift?

The following definition comes from the online book “Mouse Genetics” by Lee Silver (1995):

 

“The constant tendency of genes to evolve even in the absence of selective forces. Genetic drift is fueled by spontaneous neutral mutations that disappear or become fixed in a population at random.”

 

This includes a variety of possible mutations including single base changes, deletions, duplications, or inversions in the DNA.

 

6. How do I detect genetic drift?

 

Detection of genetic drift requires comparison of whole genome sequence data; this is typically not a cost-effective option for most labs. Drift cannot be detected using Single Nucleotide Polymorphism (SNP) scans.

 

Many times drift is detected through the observation of phenotypic differences; however, often drift does not produce a visible or detectable phenotype and may go undetected.

 

7. How do I prevent genetic drift?

 

Genetic drift is a spontaneous biological process that cannot be stopped. The potential deleterious impacts of drift on research can be minimized through the following practices:

 

  • Maintain pedigrees lines and detailed colony records
  • Watch for phenotypic changes in mutants and controls
  • Refresh breeders frequently (~every 10 generations)
  • Avoid selection pressure (choose breeders at random)
  • Cryopreserve unique strains

8. What is the difference between C57BL/6J and C57BL/6N? Or BALB/c and BALB/cByJ? What are substrains?

A substrain is a strain that

9. Do I really need to backcross my mutant to the inbred line to “refresh” it?

Genetic drift is an issue with mutant mouse strains. Refreshing your colony by backcrossing for a couple of generations to the parent inbred strain, or ordering new breeders from the vendor, can reduce the number of spontaneous mutations that may have accumulated

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B. Strain Background and Genetic Drift FAQ-Related Articles and Resources

 

1. Link to On-Demand Webinar on Achieving Reproducible Mouse Studies

2. Information on the Patented Genetic Stability Program (GSP)

3. Why it Took 2 Years for a Harvard Research Lab to Get Back to Research (Genetic Drift article)

4. Three Ways a B6J Mouse Differs from B6N and Why it Should Matter to Your 5. Research

5. BALB/cJ and BALB/cByJ: How Can I Choose? I’m So Confused!

6. There is no such thing as a C57BL/6 mouse!

7. How substrains arise

8. A tool for telling apart C57BL/6J versus C57BL/6N substrains


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