Phenotypes of Ldlr and Apoe Knockout Mice for Cardiovascular Disease Research

 

When fed a standard chow diet, 8-week-old mice with Apoe (B6.129P2-Apoetm1Unc/J, 002052) and Ldlr (B6.129S7-Ldlrtm1Her/J, 002207) mutations develop a spectrum of cardiovascular phenotypes that include:

  • Greatly elevated serum cholesterol in male and female Apoe and Ldlr knockout mice
  • Increased HDL, LDL, triglycerides, and ApoB in male and female Ldlr knockout mice
  • Male Apoe knockout mice have elevated triglycerides

The Mouse Phenome Database (MPD) contains the complete data (Jaxpheno18 dataset), including body weights, hematology at 8 weeks of age, and serum chemistries at 6, 10, 12 and 14 weeks. Mice were fed LabDiet® 5K52 formulation (6% fat) diet. Additional data in MPD (Jaxpheno10 dataset) includes cardiac pathology images and chemistries at older ages.

Clinical Chemistry. Values represent mean and one standard deviation of 10 non-fasted Apoe or Ldlr knockout mice and 5 C57BL/6J per sex. Units are mg/dL for all measurements except for free fatty acids, which are expressed as mEq/L. Results were obtained using serum from submandibular blood and a Beckman Coulter AU680 chemistry analyzer. Results were compared separately by strain and sex to C57BL/6J control mice using two-way ANOVA with Sidak’s multiple comparisons test to identify values that differed significantly, using GraphPad Prism version 7.04 for Windows (GraphPad Software).

  Apoe-null Ldlr-null C57BL/6J
  Females Males Females Males Females Males
Cholesterol 470 ± 45a 531 ± 42a 322 ± 31a 310 ± 29a 93 ± 21 126 ± 18
LDL 33 ± 10 29 ± 19 88 ± 7a 65 ± 11a 6 ± 1 4 ± 1
HDL 56 ± 8 88 ± 12 112 ± 8a 136 ± 6a 64 ± 17 87 ± 9
Glucose 153 ± 17 148 ± 23 160 ± 20 182 ± 17 149 ± 17 163 ± 24
Triglycerides 171 ± 31 239 ± 62a 195 ± 28a 206 ± 26b 145 ± 31 169 ± 31
Apo A1 1.5 ± 0.7 6.4 ± 2.5 8.7 ± 0.6 13.2 ± 1.2 9.0 ± 1.8 12.7 ± 1.5
Apo B 16.7 ± 3.6 15.7 ± 3.9 36.3 ± 4.2c 37.7 ± 4.0c 9.2 ± 2.0 11.0 ± 1.2
Free Fatty Acids 2.9 ± 0.3 2.9 ± 0.4 2.5 ± 0.3 2.3 ± 0.2 2.5 ± 0.4 2.4 ± 0.4
aP < 0.0001, bP < 0.001, cP < 0.05 compared to C57BL/6J mice of the same sex