Mice homozygous for the Ldlrtm1Her mutation have an elevated serum cholesterol level of 200-400 mg/dl and they have very high levels (>2,000 mg/dl) when fed a high fat diet. Normal serum cholesterol in the mouse is 80-100 mg/dl.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ldlr | low density lipoprotein receptor |
Mice homozygous for the Ldlrtm1Her mutation have an elevated serum cholesterol level of 200-400 mg/dl and they have very high levels (>2,000 mg/dl) when fed a high fat diet. Normal serum cholesterol in the mouse is 80-100 mg/dl.
The Ldlrtm1Her mutant strain was developed in the laboratory of Dr. Robert Hammer and Dr. Joachim Herz at the Howard Hughes Medical Institute Research Laboratories, University of Texas Southwestern Medical Center at Dallas. Insertion of a neomycin resistance cassette into exon 4 is predicted to encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. The 129-derived AB1 ES cell line was used. The strain has been backcrossed to C57BL/6J mice for 10 generations (N10).
Allele Name | targeted mutation 1, Joachim Herz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | LDLR-; LDLR KO; LDLr0; LDLrKO; LDLr-KO; Ldlrtm1Her |
Gene Symbol and Name | Ldlr, low density lipoprotein receptor |
Gene Synonym(s) | |
Site of Expression | Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 9 |
General Note | When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) Phenotypic Similarity to Human Syndrome: Atherosclerosis, Susceptibility to J:29950, J:225091 in mice fed a high-cholesterol diet. |
Molecular Note | Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
This Ldlrtm1Her strain is maintained by mating homozygous siblings. Only homozygous mice may be ordered.
When using the Ldlr KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002207 in your Materials and Methods section.
Service/Product | Description | Price |
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Homozygous for Ldlr<tm1Her> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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