CHMP2B I29V REV/WT

Product Code: JIPSC1078

Background

Genetics

Gene

CHMP2B

Condition

Amyotrophic lateral sclerosis_ PMA variant

Allele Charactertistics

Autosomal Dominant

View Information in ClinVar

Origin of Revertant

SNV/WT

Genomic Features

Genomic Coordinate (GRCh38)

chr3:87240749 (+)

dbSNP

rs63750818

Genomic Sequence

CGAGAGTTACGAGGTACACAGAGGGCTATA[A/G]TCAGAGATCGAGCAGCTTTAGAGAAACAAG
View With Genome Browser

Gene Editing Reagents

Nuclease

SpCas9

Protospacer Sequence

AGAGTTACGAGGTACACAAA

Donor Template

CGTTCTACTTACCAGCTGTTTTTCTTGTTTCTCTAAAGCTGCTCGATCTCTGATTATAGCCCTCTGTGTACCTCGTAACTCTCGATTCTGTTCCTTTATT

DNA Primers for Sequence Validation

PCR Forward

CACAGAATCCCAATAAAAGTCAGCA

PCR Reverse

GCGCCCAGCCAATATAAGAT

Sanger Sequencing Primer