Syngeneic Studies

A syngeneic mouse model, also known as an allograft mouse tumor system, provides an effective approach for studying how cancer therapies perform in the presence of a functional immune system.

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Example Study

 

Experimental Design

  • Subcutaneous engraftment into 6-8 week old mice of either CT26 (Balb/cJ) or B16-F10 (C57BL/6J) backgrounds
  • 5 study groups (n=10 per group):
    • Vehicle, positive control (JAX provides), and three test groups (2 compounds+1 combo, 3 compounds or other permutations)
    • Dosing up to 2x per week for up to 28 days
    • Body weight/clinical observations/tumor volume measurements up to 3x per week

Deliverables

  • Study report
  • Terminal blood
  • Tumor collection

Add-ons

  • FACS analysis (blood, spleen, lymph nodes, tumor)
  • Histology

Example Study: Anti-Mouse PD1/CTLA4 in CT26 engrafted Balb/cJ

Mice: Female BALB/cJ at ~6 weeks of age

Cells: CT26 cells subcutaneously injected at 2.5x106 cells per mouse

Dosing: Mice were grouped into four groups: IgG control (n=10), Anti PD-1 (n=9), Anti CTLA-4 (n=10), and Anti PD-1 +Anti CTLA-4 (n=10). Mice were IP dosed as follows:

 

Measurements: Body weight/clinical observations/tumor volume measurements 2X-3X per week

Duration: Mice were taken down when tumor volume reached 2000mm3 or when the tumor became ulcerated

Study Results: Anti-Mouse PD1/CTLA4 in CT26 Engrafted Balb/cJ – Tumor Volume