B6EiC3Sn a/A-Gy/J mice (stock number: 000503)
Genotyping protocol for Gy mice (August, 2001)
The Jackson Laboratory utilizes visible phenotype to genotype animals from the B6EiC3Sn-a/A-Gy (Stock Number 000503) colony. Animals carrying the allele display a circling behavior whereas animals do not.
The following reference provides PCR assay information that may be used to genotype animals. We do not have experience using the protocols, but offer them as potential alternatives to phenotyping methods.
Strom TM, Francis F, Lorenz B, Boddrich A, Econs MJ, Lehrach H, Meitinger T. 1997. Pex gene deletions in Gy and Hyp mice provide mouse models for X-linked hypophosphatemia. Hum Mol Genet 6:165-171.
B6.Cg-PhexHyp/J (stock number: 000528)
The Jackson Laboratory used to use visible phenotype to genotype animals from the B6.Cg-PhexHyp/J (stock number 000528) colony. Animals carrying the PhexHyp allele have reduced body size with shortened hind limbs and tail whereas animals with the Phex wild type allele do not.
Lepob mice (stock numbers: 000632 and 000696)
The Jackson Laboratory utilizes a combination of breeding scheme and visible phenotype to genotype animals from its B6.Cg-Lepob (stock number 000632) and BKS.Cg-Lepob/+ (stock number 000696) colonies.
A cross between B6129PF1-Aw-J/Aw females transplanted with Lepob/Lepob homozygous ovaries and C57BL/6J males produces heterozygous offspring. Homozygous (Lepob/Lepob) progeny from matings of these Lepob/Lep+ heterozygotes are identified phenotypically by their obesity as early as ~4 weeks of age.
Lep ob genotyping protocols:
- Lep ob, end point analysis
- Lepob, pyrosequencing
- Lepob, restriction enzyme digest