Peroxisomes are metabolic organelles that play essential roles in the functions and development of all organ systems.
In mammals, peroxisomes are responsible for the catabolism of branched and very long chain fatty acids, hydrogen peroxide, polyamines, D-amino acids, and glyoxylate and are required for the biosynthesis of plasmalogens, platelet activating factor, docosahexaenoic acid, and bile acids. The Mouse Peroxisome Research Resource (MPRR) at The Jackson Laboratory serves as a community-driven resource for mouse models and monoclonal antibodies for basic and translational research in peroxisome biology that focuses on genes encoding peroxisomal membrane and matrix proteins. The aims of the MPRR are to:
To consolidate, phenotype, distribute, and cryopreserve existing mouse models for peroxisome research. The MPRR will standardize genetic backgrounds, establish mutant alleles onto an inbred genetic background when strains are imported from the scientific community and ensure all models are cryopreserved. The MPRR leverages the phenotyping modalities and pipelines at JAX and the expertise of the Kennedy Krieger Institute Peroxisomal Diseases Laboratory to analyze peroxisomal metabolites in specimens. Lines will be deposited at the Mouse Mutant Resource and Research Center (MMRRC) at JAX for distribution.
To develop, phenotype, distribute, and cryopreserve novel mouse models for peroxisome research. The genome-editing based pipeline at JAX to generate models on a standardized C57BL/6J genetic background. Standardized phenotyping modalities and the biochemical profiling of peroxisomal metabolites will be conducted.
To develop a monoclonal antibody resource to maximize the impact of MPRR mouse models. Monoclonal antibodies will be validated by Western blot analysis and, upon request, immunostaining of cells and tissues from knockout and conditional knockout mice and in human cells. JAX will distribute the monoclonal antibodies and their characterization data.