Blood, Tissues and Organs Order Form

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To place an order using a new credit card, please contact Customer Service at 800-422-6423 or 1-207-288-5845 to have your account updated.

Order Details

You can look up strain information here: https://mice.jax.org

Service Requested:

Blood OrderTissue Order

Blood Order

Euthanasia

The standard method of euthanasia is carbon dioxide asphyxiation for mice over eight days of age.

Decapitation must be used for embryos that are E15 or older, or pup eight days of age (PND8) and under.

Organization of blood product:

Specify the volume needed, in milliliters. JAX will estimate the number of mice required and order accordingly.

Note: The amount of blood in a mouse varies by body size, age, sex, and mutation. The amount that can be obtained varies by method, with the most obtained by cardiac stick. Typically, one young adult mouse can supply 0.5-0.7 mls of whole blood, and 0.2-0.25 mls of serum or plasma, by cardiac stick. More blood is obtained from older mice, and from male mice.

Specify the number of mice needed.

Strain Name:

Stock Number:

Sex:

Genotype:

Age:

Please include days, weeks, months.

Experimental Purpose

Cell CultureDNA extractionHistologyRNA extractionProtein AnalysisOtherTo insure proper handling of these biological tissues, please indicate the purpose of this tissue order

Please Specify

Storage of blood product, prior to shipment

Aliquots

Please specify desired number and volume for aliquots:

Product Desired:

Anticoagulant

Please Specify

Collection Procedure:
The mice are euthanized by CO2 (carbon dioxide) asphyxiation and the blood is collected via a cardiac puncture. A new sterile needle and syringe are used for each mouse. Blood for sera or plasma is placed in an appropriate centrifuge tube and kept at room temperature for 20-30 minutes before being centrifuged. The blood product is placed in an appropriate collection tube for the type of product requested for storage and shipment as requested below.

Special Instructions:

Tissue Order

Tissue Type:

Number of Mice:

Strain Name:

Stock Number:

Sex:

Genotype:

Age:

Please include days, weeks, months.

Experimental Purpose

Cell CultureDNA extractionHistologyRNA extraction*Protein AnalysisOtherTo insure proper handling of these biological tissues, please indicate the purpose of this tissue order

* Consider RNAlater below for an additional charge

Please specify

Euthanasia

The standard method of euthanasia is carbon dioxide asphyxiation

for mice over eight days of age. Cervical dislocation is

an alternative method for mice greater than 14 days of age.

Decapitation must be used for embryos that are E15 or older,

or pup eight days of age (PND8) and under.

Standard method (CO2)Cervical dislocation (not appropriate for blood collection)Decapitation for mice between E15 and PND8PerfusionOther

Please specify

Perfusion
Perfusion is optional and can be done after CO2 euthanasia or

after anesthesia. The initial perfusion is with PBS to clear the

blood, followed by perfusion with fixative if needed. (Optimum

perfusion is achieved in anesthetized mice perfused with heparinized

PBS.) If blood is needed it will be drawn before the

mouse is perfused.

CO2 euthanasia followed by PerfusionAnesthesia with ip Tribromoethanol, followed by PerfusionAnesthesia with inhalant Isoflurane, follow by PerfusionNone

Perfusate

PBSAdd Heparin10% NBF4% PFA

Organization of organs

Place each organ from each mouse in a separate container.Pool different organs from the one mouse in the same containerPool same organs from different mice in the same containerOther

Please specify

Collection and storage of organs

Freeze in liquid nitrogen or on dry ice. Store at -80 degrees Celsius.Collect on cold packs. Store at 4 degrees (not applicable to international shipments)AmbientOther: Specify special fixatives/reagents/media

Please specify

Special Fixatives/Reagents

10% NBF4% PFABouin’sRNAlater*PBSOther* RNAlater can be kept at any temperature. If -80 is desired, the manufacturer recommendation is to place it at 4C overnight before transfer to -80

Please specify

Tissue Culture Media
(requires minimum of 2-day shipping)

Advanced DMEMAdd 10% FBSAdd 1% Pen/StrepRPMI 1640Other

Please specify

Additional Requests

NoneTissue Weights - all organs collected.Tissue Weights - Other
(Please specify in Special Instructions)Additional Tissue Cuts
(Please specify which organ, number of cuts, etc. in the Special Instructions)Flush organ with solution to clear contents (e.g. GI tract, heart) to inflate (lungs), or instill solution for quicker fixation. Specify which organs and which solution in the special instructions

Tissue Block

Paraffin BlockCryo Block

Tissue Orientation within block

Cross / Transverse SectionLongitudinal / SagittalOther

Please specify

Special Instructions:

Pre - Ship Storage

-80º C*4º CAmbient

Shipping Method

Dry Shipper
(LN2 vapor, for international shipments only)Dry ice
(not applicable to international shipments)Cold packs
(not applicable to international shipments)Ambient temperature
(not applicable for blood products)

Important Notes:

1. Cancellation Policy: This is a special order and will be subject to a cancellation fee. For precise details please visit our Purchase Policies - Cancelation, Order Changes, and Credit page.

2. The price of this service does not include the cost of the mice needed to perform the service. The cost of the mice is additional.