Distribution of genetically defined mouse embryonic stem (mES) cells from gold standard JAX® Mice.
| Strain number Product name |
Passage number |
Strain of origin | Availability |
|---|---|---|---|
| 10 |
B6(Cg)-Tyrc-2J/J |
In stock |
|
|
000486 |
9 |
MRL/MpJ |
In stock |
|
000646 |
9 |
A/J |
In stock |
|
000651 |
12 |
BALB/cJ |
In stock |
| 10 |
BALB/cJ |
In stock |
|
|
000659 |
10 |
C3H/HeJ |
In stock |
|
000659 |
10 |
C3H/HeJ |
In stock |
| 11 |
C3H/HeJ |
In stock |
|
|
000671 |
12 |
DBA/2J |
In stock |
|
000671 |
11 |
DBA/2J |
In stock |
| 10 |
129X1/SvJ |
In stock |
|
| 9 |
CAST/EiJ |
In stock |
|
| 9 |
WSB/EiJ |
In stock |
|
|
001026 |
8 |
BALB/cByJ |
In stock |
| 10 |
BALB/cByJ |
In stock |
|
|
001058 |
9 |
NZW/LacJ |
In stock |
| 10 |
C3Fe.SWV-Mbpshi/J |
In stock |
|
| 10 |
C3Fe.SWV-Mbpshi/J |
In stock |
|
| 10 |
C3Fe.SWV-Mbpshi/J |
In stock |
|
|
001800 |
9 or 10 |
FVB/NJ |
In stock |
| 5 |
NOD/ShiLtJ |
In stock |
|
| 9 |
NZO/HiltJ |
In stock |
|
| 9 |
BTBR T+ Itpr3tf/J |
In stock |
|
| 9 |
129S1/SvImJ |
In stock |
|
| 8 |
NOD.CBALs-Tyr+/LtJ |
In stock |
|
| 8 | C57BL/6J-Chr 1A/J/NaJ |
In stock |
|
| 7 | C57BL/6J-Chr 2A/J/NaJ |
In stock |
|
| 8 | C57BL/6J-Chr 3A/J/NaJ |
In Stock |
|
| -- |
C57BL/6J-Chr 6A/J/NaJ |
In Stock |
|
| -- |
C57BL/6J-Chr 9A/J/NaJ |
In Stock |
|
| -- |
C57BL/6J-Chr 10A/J/NaJ |
In stock |
|
| -- |
C57BL/6J-Chr 11A/J/NaJ |
In stock |
|
| 8 | C57BL/6J-Chr 12A/J/NaJ |
In stock |
|
| 7 |
PWD/PhJ |
In stock |
|
| 11 | C57BL/6N
|
In stock |
|
|
017529 |
27 | 129S2/SvPas |
In stock |
Please see our article on this topic, Germline competency: what does it mean and how is it determined?
B6-693 ES cells proved germline competent in six experiments at passages 13, 15, 19, 31 and 32. Four of the six experiments were following ES cell targeting. Chimeric mice were obtained by unilateral transfer of 10-12 microinjected blastocysts to the uteri of ~10 pseudopregnant female mice for each experiment. In six experiments with B6 mES cells, the number of coat-color chimeras divided by the number of mice born were 10/66, 4/13, 6/15, 5/20, 21/59, 10/39. Additional information can be found in the tech sheet for this cell line, but this ES Cell line is no longer available.
We first distributed the B6-693 mES cells at P16. The batch was expanded from a starting population of ES cells at P12. We generated a limited number of vials at P13 and P14 for seed stock and then expanded to P16 to obtain sufficient quantities of the sellable stock. We subsequently distributed B6-693 mES cells at P11 that were generated with cells from a starting population at P9. That P11 line was karyotyped male with high percentage of normal spreads.
mES cells at an earlier passage number typically have higher frequencies of germline transmission and a lower likelihood of chromosomal gains, losses, or translocations (i.e. more likely to have a normal karyotype). In general, the more passages an ES cell line is subjected to, the greater the chance of changes in karyotype that could negatively impact their ability to create germline chimeras.
The MEK inhibitor was included to ensure that cells remained undifferentiated during expansion.
The ES cell lines created and characterized by Deltagen Inc. are available from The Jackson Laboratory*. Companion mouse strains created using Deltagen’s ES cell lines are also available via The Jackson Laboratory Repository.
*These products became available after 2005, when the NIH announced an agreement allowing the distribution of mutant mice and frozen embryos from two private collections of knockout (KO) mice.
Or contact customer service at 1-800-422-6423 (US, Canada & Puerto Rico) or 1-207-288-5845 (from any location).
Representatives are available Monday-Friday 8am-8pm (ET) / 5am-5pm (PT).
| Item number | ES | Gene name | Stock number of companion strain |
|---|---|---|---|
|
009127C01 |
EC-Htr4tm1Dgen |
5 hydroxytryptamine (serotonin) receptor 4 |
005767 |
|
009128C01 |
EC-Htr5atm1Dgen |
5-hydroxytryptamine (serotonin) receptor 5A |
005768 |
|
009129C01 |
EC-Htr7tm1Dgen |
5-hydroxytryptamine (serotonin) receptor 7 |
005769 |
|
009130C01 |
EC-Adamts4tm1Dgen |
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 |
005770 |
|
009164C01 |
EC-Adamts5tm1Dgen |
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) |
005771 |
|
009162C01 |
EC-Acvrl1tm1Dgen |
activin A receptor, type II-like 1 |
005772 |
|
009165C01 |
EC-Adcy3tm1Dgen |
adenylate cyclase 3 |
005773 |
|
009166C01 |
EC-Adcy7tm1Dgen |
adenylate cyclase 7 |
005774 |
|
009167C01 |
EC-Adipor2tm1Dgen |
adiponectin receptor 2 |
005775 |
|
009168C01 |
EC-Avpr1atm1Dgen |
arginine vasopressin receptor 1A |
005776 |
|
009169C01 |
EC-Axltm1Dgen |
AXL receptor tyrosine kinase |
005777 |
|
009170C01 |
EC-Birc1atm1Dgen |
baculoviral IAP repeat-containing 1a |
005778 |
|
009183C01 |
EC-Celsr2tm1Dgen |
cadherin EGF LAG seven-pass G-type receptor 2 |
005779 |
|
009173C01 |
EC-Cacna2d3tm1Dgen |
calcium channel, voltage-dependent, alpha2/delta subunit 3 |
005780 |
|
009174C01 |
EC-Cacng3tm1Dgen |
calcium channel, voltage-dependent, gamma subunit 3 |
005781 |
|
009175C01 |
EC-Cacng4tm1Dgen |
calcium channel, voltage-dependent, gamma subunit 4 |
005782 |
|
009171C01 |
EC-Cacna1ctm1Dgen |
calcium channel, voltage-dependent, L type, alpha 1C subunit |
005783 |
|
009176C01 |
EC-Capn5tm1Dgen |
calpain 5 |
005784 |
|
009177C01 |
EC-Capn7tm1Dgen |
calpain 7 |
005785 |
|
009186C01 |
EC-Cnr2tm1Dgen |
cannabinoid receptor 2 (macrophage) |
005786 |
|
009187C01 |
EC-Ctsctm1Dgen |
cathepsin C |
005787 |
|
009182C01 |
EC-Cd97tm1Dgen |
CD97 antigen |
005788 |
|
009184C01 |
EC-Cer1tm1Dgen |
cerberus 1 homolog (Xenopus laevis) |
005789 |
|
009247C01 |
EC-Tpp1tm1Dgen |
ceroid-lipofuscinosis, neuronal 2 |
005790 |
|
009238C01 |
EC-Xcr1tm1Dgen |
chemokine (C motif) receptor 1 |
005791 |
|
009178C01 |
EC-Ccr1l1tm1Dgen |
chemokine (C-C motif) receptor 1-like 1 |
005792 |
|
009179C01 |
EC-Ccr6tm1Dgen |
chemokine (C-C motif) receptor 6 |
005793 |
|
009180C01 |
EC-Ccr7tm1Dgen |
chemokine (C-C motif) receptor 7 |
005794 |
|
009181C01 |
EC-Ccrl2tm1Dgen |
chemokine (C-C motif) receptor-like 2 |
005795 |
|
009188C01 |
EC-Cxcr3tm1Dgen |
chemokine (C-X-C motif) receptor 3 |
005796 |
|
009185C01 |
EC-Chrna2tm1Dgen |
cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) |
005797 |
|
009189C01 |
EC-Drd5tm1Dgen |
dopamine receptor 5 |
005798 |
|
009190C01 |
EC-S1pr4tm1Dgen |
endothelial differentiation, G-protein-coupled receptor 6 |
005799 |
|
009191C01 |
EC-Efemp2tm1Dgen |
epidermal growth factor-containing fibulin-like extracellular matrix protein 2 |
005800 |
|
009241C01 |
EC-Esrratm1Dgen |
estrogen related receptor, alpha |
005801 |
|
009192C01 |
EC-Faim2tm1Dgen |
Fas apoptotic inhibitory molecule 2 |
005802 |
|
009193C01 |
EC-Fzd1tm1Dgen |
frizzled homolog 1 (Drosophila) |
005803 |
|
009194C01 |
EC-Fzd8tm1Dgen |
frizzled homolog 8 (Drosophila) |
005804 |
|
009199C01 |
EC-Gpr151tm1Dgen |
G protein-coupled receptor 151 |
005805 |
|
009200C01 |
EC-Gpr37tm1Dgen |
G protein-coupled receptor 37 |
005806 |
|
009201C01 |
EC-Gpr6tm1Dgen |
G protein-coupled receptor 6 |
005807 |
|
009202C01 |
EC-Gprk5tm1Dgen |
G protein-coupled receptor kinase 5 |
005808 |
|
009242C01 |
EC-Galr1tm1Dgen |
galanin receptor 1 |
005809 |
|
009197C01 |
EC-Gabrptm1Dgen |
gamma-aminobutyric acid (GABA-A) receptor, pi |
005810 |
|
009195C01 |
EC-Gabra3tm1Dgen |
gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 3 |
005811 |
|
009196C01 |
EC-Gabra4tm1Dgen |
gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4 |
005812 |
|
009203C01 |
EC-Grik5tm1Dgen |
glutamate receptor, ionotropic, kainate 5 (gamma 2) |
005813 |
|
009204C01 |
EC-Grm1tm1Dgen |
glutamate receptor, metabotropic 1 |
005814 |
|
009205C01 |
EC-Grm3tm1Dgen |
glutamate receptor, metabotropic 3 |
005815 |
|
009198C01 |
EC-Glra3tm1Dgen |
glycine receptor, alpha 3 subunit |
005816 |
|
009206C01 |
EC-Gsk3btm1Dgen |
glycogen synthase kinase 3 beta |
005817 |
|
009207C01 |
EC-Hcrtr1tm1Dgen |
hypocretin (orexin) receptor 1 |
005818 |
|
009209C01 |
EC-Insl5tm1Dgen |
insulin-like 5 |
005819 |
|
009208C01 |
EC-Il8ratm1Dgen |
interleukin 8 receptor, alpha |
005820 |
|
009211C01 |
EC-Lats2tm1Dgen |
large tumor suppressor 2 |
005821 |
|
009212C01 |
EC-Lmbr1tm1Dgen |
limb region 1 |
005822 |
|
009213C01 |
EC-Lrp5tm1Dgen |
low density lipoprotein receptor-related protein 5 |
005823 |
|
009215C01 |
EC-Mmp17tm1Dgen |
matrix metalloproteinase 17 |
005824 |
|
009216C01 |
EC-Mtmr1tm1Dgen |
myotubularin related protein 1 |
005825 |
|
009217C01 |
EC-Ntsr1tm1Dgen |
neurotensin receptor 1 |
005826 |
|
009220C01 |
EC-Ptch2tm1Dgen |
patched homolog 2 |
005827 |
|
009243C01 |
EC-Ppardtm1Dgen |
peroxisome proliferator activator receptor delta |
005828 |
|
009218C01 |
EC-Pkd2l2tm1Dgen |
polycystic kidney disease 2-like 2 |
005829 |
|
009210C01 |
EC-Kcnq2tm1Dgen |
potassium voltage-gated channel, subfamily Q, member 2 |
005830 |
|
009219C01 |
EC-Ppm1ftm1Dgen |
protein phosphatase 1F (PP2C domain containing) |
005831 |
|
009221C01 |
EC-Ptprotm1Dgen |
protein tyrosine phosphatase, receptor type, O |
005832 |
|
009222C01 |
EC-Rgs4tm1Dgen |
regulator of G-protein signaling 4 |
005833 |
|
009225C01 |
EC-Sema5atm1Dgen |
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A |
005834 |
|
009226C01 |
EC-Sema6ctm1Dgen |
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C |
005835 |
|
009224C01 |
EC-Scn9atm1Dgen |
sodium channel, voltage-gated, type IX, alpha |
005836 |
|
009223C01 |
EC-Scn11atm1Dgen |
sodium channel, voltage-gated, type XI, alpha |
005837 |
|
009228C01 |
EC-Slc22a6tm1Dgen |
solute carrier family 22 (organic anion transporter), member 6 |
005838 |
|
009227C01 |
EC-Slc22a12tm1Dgen |
solute carrier family 22 (organic anion/cation transporter), member 12 |
005839 |
|
009230C01 |
EC-Slc6a9tm1Dgen |
solute carrier family 6 (neurotransmitter transporter, glycine), member 9 |
005841 |
|
009231C01 |
EC-Slc7a8tm1Dgen |
solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 |
005842 |
|
009232C01 |
EC-Slc9a6tm1Dgen |
solute carrier family 9 (sodium/hydrogen exchanger), isoform 6 |
005843 |
|
009233C01 |
EC-Sstr1tm1Dgen |
somatostatin receptor 1 |
005844 |
|
009235C01 |
EC-Thbs4tm1Dgen |
thrombospondin 4 |
005845 |
|
009245C01 |
EC-Tlr2tm1Dgen |
toll-like receptor 2 |
005846 |
|
009234C01 |
EC-Tgfbr1tm1Dgen |
transforming growth factor, beta receptor I |
005847 |
|
009237C01 |
EC-Trpm5tm1Dgen |
transient receptor potential cation channel, subfamily M, member 5 |
005848 |
|
009236C01 |
EC-Tmprss11atm1Dgen |
transmembrane protease, serine 11a |
005849 |
|
009214C01 |
EC-Mapkapk2tm1Dgen |
MAP kinase-activated protein kinase 2 |
005850 |
|
009239C01 |
EC-Adam21tm1Dgen |
mitogen-activated protein kinase-activated protein kinase 2 |
006431 |
|
009244C01 |
EC-Slc18a1tm1Dgen |
solute carrier family 18 (vesicular monoamine), member 1 |
006432 |
|
009240C01 |
EC-Atp8a1tm1Dgen |
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 |
006433 |
|
009246C01 |
EC-Tmprss13tm1Dgen |
transmembrane protease, serine 13 |
006434 |
Research institutions require an Academic Use License, and companies require a license prior to shipping.
This mouse strain (including any and all progeny, "MICE") is distributed by The Jackson Laboratory with the following terms and conditions.
MATERIALS ARE AVAILABLE TO NONPROFIT RESEARCH INSTITUTIONS, UNIVERSITIES AND HOSPITALS THAT HAVE AN ACADEMIC LICENSE AGREEMENT WITH DELTAGEN INC. COMPANIES REQUIRE A SEPARATE COMMERCIAL USE LICENSE WITH DELTAGEN. AN ACADEMIC LICENSE AGREEMENT WILL BE FORWARDED FOR APPROVAL THE FIRST TIME A MATERIAL IS ORDERED.
MATERIALS may be sent by the NIH-designated repositories to any
academic or nonprofit organization worldwide that have signed the Academic
License with Deltagen, but may not be sent to commercial organizations
without a license. The only restriction on the use of the mice or derived
materials in academic research is that they may not be used for industry-funded
contract research. There are no publishing or intellectual property restrictions
on the use of the mouse materials. Researchers are free to publish and
seek patents on results obtained using the mouse materials without restriction.
Robert Driscoll, J.D., Ph.D.
President and Chief Executive Officer
Deltagen, Inc.
Tel: 650-345-7602
Email: rdriscoll@deltagen.com
1900 S. Norfolk St., Suite 105
San Mateo, CA 94403
The Jackson Laboratory Contracts Administration
Tel:
207-288-6470
Email: tjlca@jax.org
610 Main Street
Bar Harbor, Maine 04609-1500