Protocol 17584: Standard PCR Assay - Laragen
Version 2.0

Notes

27410 expected CAG size is between 180 and 200
For additional information please contact Laragen directly:
Jin Li
Laragen, Inc.
10601 Virginia Ave. 
Culver City,  CA 90232
310 280 0804
jin@laragen.com
www.laragen.com
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

27410 expected CAG size is between 180 and 200

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note

Strains Using This Protocol

Stock Number Strain Name
035806 129S4/SvJae-Fgfr2tm6.1Sor/Mmjax
003598 B6.129-Htttm5Mem/J
035500 B6.129-Pick1tm1.1Rlh/J
034226 B6.129-Rab25tm1Jrgo/J
004595 B6.129P2-Htttm2Detl/150J
021193 B6.129P2-Htttm2Detl/365J
034729 B6.129P2-Myliptm1Pton/J
035533 B6.129P2-Sntb2tm1Scf/J
005601 B6.129S-Atxn1tm1Hzo/J
034595 B6.129S-Capn1tm1Ahc/J
027411 B6.129S1(Cg)-Htttm2Mem/20ChdiJ
035475 B6.129S1-Ifi30tm1Cres/J
027412 B6.129S4-Htttm1.1Pfs/80ChdiJ
035514 B6.Cg-Cd19tm1(cre)Cgn Ikzf3tm1.1Cjwu/J
012329 B6.Cg-Fxntm1.1Pand Fxntm1Mkn/J
012910 B6.Cg-Fxntm1Mkn Tg(FXN)YG22Pook/J
027430 B6.Cg-Gt(ROSA)26Sortm47(HTT)Arte/ChdiJ
027429 B6.Cg-Gt(ROSA)26Sortm48(HTT)Arte/ChdiJ
027428 B6.Cg-Gt(ROSA)26Sortm49(HTT)Arte/ChdiJ
027427 B6.Cg-Gt(ROSA)26Sortm50(HTT)Arte/ChdiJ
027426 B6.Cg-Gt(ROSA)26Sortm51(HTT)Arte/ChdiJ
027431 B6.Cg-Gt(ROSA)26Sortm52(HTT)Arte/ChdiJ
027435 B6.Cg-Htttm1(HTT)Arte/ChdiJ
007001 B6.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/1J
008085 B6.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/2J
029436 B6.Cg-Tg(CMV-AR*113Q)FMonk/J
006471 B6.Cg-Tg(HDexon1)61Gpb/J
033705 B6.Cg-Tg(Lck-RHO/ADRA1,-lacZ)B22-1Mik/J
027432 B6.FVB-Tg(YAC128)53Hay/ChdiJ
033109 B6;129-Cdh2tm1(cre/ERT)Linli/J
035714 B6;129S1-Gt(ROSA)26Sortm1(Bcor*A)Vjba/J
003284 B6;129S1-Il1raptm1Roml/J
012705 B6;CBA-Tg(ATXN3*)84.2Cce/IbezJ
002810 B6CBA-Tg(HDexon1)62Gpb/1J
006494 B6CBA-Tg(HDexon1)62Gpb/3J
029928 B6J.129S1-Htttm1.1Mfc/190ChdiJ
027409 B6J.129S1-Htttm1Mfc/140ChdiJ
027410 B6J.129S1-Htttm1Mfc/190ChdiJ
370476 B6J.129S1-Htttm1Mfc/190JChdi
027419 B6J.Cg-Tg(HDexon1)62Gpb/110ChdiJ
027421 B6J.Cg-Tg(HDexon1)62Gpb/160ChdiJ
034731 B6N(129S4)-Srd5a3tm1c(EUCOMM)Wtsi/J
034643 B6N.129-Irgm1tm1Gat/J
032924 B6N.129X1(B6J)-Msratm1Jmo/RlevMmjax
027413 B6N.Cg-Htttm1Xen/45ChdiJ
027414 B6N.Cg-Htttm2Xen/45ChdiJ
027415 B6N.Cg-Htttm3Xen/80ChdiJ
027416 B6N.Cg-Htttm4Xen/80ChdiJ
027417 B6N.Cg-Htttm5Xen/105ChdiJ
027418 B6N.Cg-Htttm6Xen/105ChdiJ
031371 C.129S6-Pglyrp3tm1Rdz/J
035489 C57BL/6-Elmo1tm1Mki/J
035698 C57BL/6-Il17atm1Bcgen Il17fem1Litt/J
003651 C57BL/6-Tg(Zp3-cre)93Knw/J
034448 C57BL/6J-Tlr9em1Ldm/J
027422 D2.Cg-Tg(HDexon1)62Gpb/160ChdiJ
027424 D2.Cg-Tg(HDexon1)62Gpb/240ChdiJ
027425 D2.Cg-Tg(HDexon1)62Gpb/280ChdiJ
004938 FVB-Tg(YAC128)53Hay/J
027379 FVB/N-Tg(HTT*)NXwy/J
008197 FVB/N-Tg(HTT*97Q)IXwy/J
027433 FVB/N-Tg(HTT*97Q)LXwy/ChdiJ
007850 J:NU
026014 NOD.Cg-Rag1tm1Mom KitW-41J Il2rgtm1Wjl/EavJ
007799 NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ
014568 NOD.Cg-Rag1tm1Mom Ins2Akita Il2rgtm1Wjl/SzJ
035185 STOCK Adgrl1tm2c(EUCOMM)Hmgu/SudJ
031007 STOCK Fxnem2.1Lutzy Tg(FXN)Y47Pook/J
030395 STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/800J
030930 STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/J
024097 STOCK Fxntm1Mkn Tg(FXN)Y47Pook/J
024113 STOCK Fxntm1Mkn Tg(FXN)YG8Pook/2J
012266 STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J
035444 STOCK Hspa5tm1.1Alee/J
003456 STOCK Htttm5Mem/J
006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
030324 STOCK Tg(FXN)YG8Pook/J
018299 STOCK Tg(FXN*)1Sars Fxntm1Mkn/J
78 strains use this protocol