| Allele Name | targeted mutation 5, Jeremy Nathans |
| Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence) |
| Allele Synonym(s) | R26-CAG-LSL-Sun1-sfGFP-Myc; SUN1-2xsfGFP-6xMYC |
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
| Gene Synonym(s) | |
| Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | Myc, myelocytomatosis oncogene, mouse, laboratory |
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| Expressed Gene | Sun1, Sad1 and UNC84 domain containing 1, mouse, laboratory |
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| Site of Expression | The SUN1 fusion protein (SUN1 fused to sfGFP) is expressed at the inner nuclear membrane in targeted cell types where promoters driving Cre recombinase are expressed. |
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| Strain of Origin | 129 |
| Chromosome | 6 |
| Molecular Note | A targeting vector containing (from 5' to 3') a CAG promoter, a loxP site flanked STOP cassette, the mouse Sun1 cDNA fused with 2 copies of the superfolder GFP (sfGFP) green fluorescent protein gene and 6 copies of the Myc tag, and an FRT site flanked neomycin resistance gene cassette was inserted into the locus. Removal of the floxed stop cassette is required to achieve expression. |
