B6;CBA-Tg(Olfr16*,taulacZ)19Mom/MomJ

Stock number: 006680
Product name: MOR23-Tg3'Δ-19-LacZ
Strain synonyms: MOR23-Tg3'Delta-IRES-tauLacZ (line Tg3'Delta)

Development

A 9.4 kb Sac1-Nhe1 fragment containing Olfr16 was subcloned from a 129/Sv-derived P1 phage into pBluescript, the first and second introns were excised, the 3? end of the insert was truncated by 1.4 kb, and an IRES-taulacZ sequence was inserted just downstream of the coding sequence. This resulted in a 2.2 kb construct that was microinjected into (C57BL/6J x CBA)F2 zygotes and approximately 3 copies of the transgene integrated by random insertion into line 19.

Allele

Symbol: Tg(Olfr16*,taulacZ)19Mom
Name: transgene insertion 19, Peter Mombaerts
MGI accession ID: MGI:3711523
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Olfr16*,taulacZ)19Mom
Marker name: transgene insertion 19, Peter Mombaerts
Chromosome: UN
Strain of origin: (C57BL/6J x CBA)F2
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in olfactory sensory neurons on the turbinate epithelium.
Molecular note: A genomic DNA containing an IRES-taulacZ 3-prime tag from which introns one and two, as well as a 14kb 3' segment, have been removed was microinjected into pronuclei of C57BL/6J X CBA F2 zygotes. This construct contains 2.2 kb of spliced genomic sequence including 0.9 kb of coding sequence, and confers basic properties of olfactory receptor gene expression such as olfactory sensory neuron-specific, punctate expression and coconvergence to cognate glomeruli.