Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
A 9.4 kb Sac1-Nhe1 fragment containing Olfr16 was subcloned from a 129/Sv-derived P1 phage into pBluescript, the first and second introns were excised, the 3? end of the insert was truncated by 1.4 kb, and an IRES-taulacZ sequence was inserted just downstream of the coding sequence. This resulted in a 2.2 kb construct that was microinjected into (C57BL/6J x CBA)F2 zygotes and approximately 3 copies of the transgene integrated by random insertion into line 19.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ is expressed in olfactory sensory neurons on the turbinate epithelium. |
Allele Name | transgene insertion 19, Peter Mombaerts |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | MOR23-Tg3'Delta-IRES-tauLacZ (line Tg3'Delta); Tg3'delta-19 |
Gene Symbol and Name | Tg(Olfr16*,taulacZ)19Mom, transgene insertion 19, Peter Mombaerts |
Gene Synonym(s) | |
Promoter | Olfr16, olfactory receptor 16, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in olfactory sensory neurons on the turbinate epithelium. |
Strain of Origin | (C57BL/6J x CBA)F2 |
Chromosome | UN |
Molecular Note | A genomic DNA containing an IRES-taulacZ 3-prime tag from which introns one and two, as well as a 14kb 3' segment, have been removed was microinjected into pronuclei of C57BL/6J X CBA F2 zygotes. This construct contains 2.2 kb of spliced genomic sequence including 0.9 kb of coding sequence, and confers basic properties of olfactory receptor gene expression such as olfactory sensory neuron-specific, punctate expression and coconvergence to cognate glomeruli. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the MOR23-Tg3'Δ-19-LacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006680 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Olfr16*,taulacZ)19Mom |
Frozen Mouse Embryo | B6;CBA-Tg(Olfr16* taulacZ)19Mom/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Olfr16* taulacZ)19Mom/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(Olfr16* taulacZ)19Mom/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(Olfr16* taulacZ)19Mom/MomJ Frozen Embryo | $3373.50 |
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