The Cdh6 gene product is a transmembrane protein that is expressed during the earliest stages of nephrogenesis. Mice that are homozygous-null for this gene are viable and fertile with no detectable gene Cdh6 product. They possess only two-thirds the normal number of formed, fully functioning nephrons seen in adult wildtype mice. This diminished number of nephrons is thought to result from delayed epithelialization in the developing kidneys.
A targeting vector containing the neomycin resistance gene and a PGK-tk cassette was used to disrupt the first exon of the Cdh6 gene in CCE embryonic stem cells. The insertion interferes with the normal functioning of the signal sequence such that any transcripts produced are not targeted to the membrane. Selected ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were bred to C57BL/6 females.
|Allele Name||targeted mutation 1, Steven P Mah|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Cad-6 -|
|Gene Symbol and Name||Cdh6, cadherin 6|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||A neomycin resistance gene was inserted into exon 1 at codon 68. Western blot analysis on embryonic kidney lysates derived from homozygous mice demonstrated that no detectable protein was produced from this allele. Immunohistochemistry expermients confirmed that homozygous mice did not express detectable levels of the encoded protein (data not shown).|
|Mutations Made By|| |
Dr. Steven Mah, Digital Gene Technologies, Inc.
This strain originated on a B6;129S background. It has been backcrossed to B6 for seven generations and is maintained by the donating investigator as a homozygote.
When using the B6.129S6-Cdh6tm1Sma/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003742 in your Materials and Methods section.