STOCK Rag1^tm1Mom Tg(TIE2GFP)287Sato/J

Stock number: 006770
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

This double mutant mouse strain possessing a Rag1 knock-out and an endothelial expressing green fluorescent protein (GFP) transgene may be useful in studies examining angiogenesis in transplanted tissues.

Detailed description

This double mutant strain incorporates Rag1^tm1Mom knock-out from the T and B-cell deficient C.129S7(B6)-Rag1^tm1Mom/J strain (Stock No. 003145) and the endothelial GFP expressing Tg(TIE2GFP)287Sato transgene from the now discontinued B6.Cg-Tg(TIE2GFP)287Sato/1J strain (Stock No, 004659 - refer to STOCK Tg(TIE2GFP)287Sato/J Stock No. 003658).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described for each single mutant. We will modify the strain description if necessary as published results become available.
Of note, this strain does not breed well on the B6 background. The single Tg(TIE2GFP)287Sato transgenic line has a lethal phenotype on the B6 background.

Development

For the Rag1^tm1Mom targeted mutation, a replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129S7/SvEvBrd AB1 ES cell line was used. The BALB/c congenic strain was generated by Dr. Bob Coffman by backcrossing mice carrying the Rag1^tm1Mom mutation seven times to BALB/cAnNTac inbred mice.

The Tg(TIE2GFP)287Sato transgenic mutation originated on an FVB/N background, and was backcrossed to C57BL/6J for five generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.

To generate this double mutant strain, C.129S7(B6)-Rag1^tm1Mom/J (Stock No. 003145) was crossed to B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659 - discontinued. See STOCK Tg(TIE2GFP)287Sato/J, Stock No. 003658).

Allele

Symbol: Tg(TIE2GFP)287Sato
Name: transgene insertion 287, Thomas N Sato
MGI accession ID: MGI:3056937
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(TIE2GFP)287Sato
Marker name: transgene insertion 287, Thomas N Sato
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS.
Molecular note: The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter.
General note: Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS.

Allele

Symbol: Rag1^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857241
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rag1
Marker name: recombination activating gene 1
Chromosome: 2
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Site of expression: expression is seen in bone marrow derived cell lines.
Molecular note: A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.