This double mutant mouse strain possessing a Rag1 knock-out and an endothelial expressing green fluorescent protein (GFP) transgene may be useful in studies examining angiogenesis in transplanted tissues.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Allele Type |
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Transgenic (Reporter) |
This double mutant strain incorporates Rag1tm1Mom knock-out from the T and B-cell deficient C.129S7(B6)-Rag1tm1Mom/J strain (Stock No. 003145) and the endothelial GFP expressing Tg(TIE2GFP)287Sato transgene from the now discontinued B6.Cg-Tg(TIE2GFP)287Sato/1J strain (Stock No, 004659 - refer to STOCK Tg(TIE2GFP)287Sato/J Stock No. 003658).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described for each single mutant. We will modify the strain description if necessary as published results become available.
Of note, this strain does not breed well on the B6 background. The single Tg(TIE2GFP)287Sato transgenic line has a lethal phenotype on the B6 background.
For the Rag1tm1Mom targeted mutation, a replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129S7/SvEvBrd AB1 ES cell line was used. The BALB/c congenic strain was generated by Dr. Bob Coffman by backcrossing mice carrying the Rag1tm1Mom mutation seven times to BALB/cAnNTac inbred mice.
The Tg(TIE2GFP)287Sato transgenic mutation originated on an FVB/N background, and was backcrossed to C57BL/6J for five generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.
To generate this double mutant strain, C.129S7(B6)-Rag1tm1Mom/J (Stock No. 003145) was crossed to B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659 - discontinued. See STOCK Tg(TIE2GFP)287Sato/J, Stock No. 003658).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion 287, Thomas N Sato |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(Tek-GFP)287Sato; TIE2-GFP |
Gene Symbol and Name | Tg(TIE2GFP)287Sato, transgene insertion 287, Thomas N Sato |
Gene Synonym(s) | |
Promoter | Tek, TEK receptor tyrosine kinase, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS. |
Strain of Origin | Not Applicable |
Chromosome | UN |
General Note | Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS. |
Molecular Note | The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter. |
Mutations Made By | Thomas Sato, Nara Institute of Science and Technology |
When maintaining a live colony, these mice can be bred as homozygous for the Rag1tm1Mom allele and homozygous for the Tg(TIE2GFP)287Sato transgene.
When using the STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006770 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rag1<tm1Mom>, Hemizygous for Tg(TIE2GFP)287Sato |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J | $2595.00 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J | $2595.00 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J | $3373.50 |
Frozen Mouse Embryo | STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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