Overview

This double mutant mouse strain possessing a Rag1 knock-out and an endothelial expressing green fluorescent protein (GFP) transgene may be useful in studies examining angiogenesis in transplanted tissues.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Rag1^tm1Mom

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Rag1	recombination activating gene 1

Tg(TIE2GFP)287Sato

Allele Type
Transgenic (Reporter)

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Cancer Research
Hematological Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

This double mutant strain incorporates Rag1^tm1Mom knock-out from the T and B-cell deficient C.129S7(B6)-Rag1^tm1Mom/J strain (Stock No. 003145) and the endothelial GFP expressing Tg(TIE2GFP)287Sato transgene from the now discontinued B6.Cg-Tg(TIE2GFP)287Sato/1J strain (Stock No, 004659 - refer to STOCK Tg(TIE2GFP)287Sato/J Stock No. 003658).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described for each single mutant. We will modify the strain description if necessary as published results become available.
Of note, this strain does not breed well on the B6 background. The single Tg(TIE2GFP)287Sato transgenic line has a lethal phenotype on the B6 background.

Development

For the Rag1^tm1Mom targeted mutation, a replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129S7/SvEvBrd AB1 ES cell line was used. The BALB/c congenic strain was generated by Dr. Bob Coffman by backcrossing mice carrying the Rag1^tm1Mom mutation seven times to BALB/cAnNTac inbred mice.

The Tg(TIE2GFP)287Sato transgenic mutation originated on an FVB/N background, and was backcrossed to C57BL/6J for five generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.

To generate this double mutant strain, C.129S7(B6)-Rag1^tm1Mom/J (Stock No. 003145) was crossed to B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659 - discontinued. See STOCK Tg(TIE2GFP)287Sato/J, Stock No. 003658).

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Genetics

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Allele Name	targeted mutation 1, Peter Mombaerts
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Rag-; Rag^1tm1Mom; RAG-1-; Rag1^-; RAG1^null; Rag-1KO
Gene Symbol and Name	Rag1, recombination activating gene 1
Gene Synonym(s)
Site of Expression	expression is seen in bone marrow derived cell lines.
Strain of Origin	129S7/SvEvBrd-Hprt⁺
Chromosome	2
Molecular Note	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Mutations Made By	Peter Mombaerts, Max Planck Research Unit for Neurogenetics

Tg(TIE2GFP)287Sato

Allele Symbol: Tg(TIE2GFP)287Sato

Allele Name	transgene insertion 287, Thomas N Sato
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	Tg(Tek-GFP)287Sato; TIE2-GFP
Gene Symbol and Name	Tg(TIE2GFP)287Sato, transgene insertion 287, Thomas N Sato
Gene Synonym(s)
Promoter	Tek, TEK receptor tyrosine kinase, mouse, laboratory
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS.
Strain of Origin	Not Applicable
Chromosome	UN
General Note	Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS.
Molecular Note	The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter.
Mutations Made By	Thomas Sato, Nara Institute of Science and Technology

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1^tm1Mom related

Immunology, Inflammation and Autoimmunity Research
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
- Immunodeficiency
  - B and T cell deficiency
- Inflammation
  - B and T cell deficiency
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
Hematological Research
- Immunological Defects
  - B and T cell deficiency

Research Tools
- Fluorescent Proteins
- Cardiovascular Research
  - endothelial cell marker for neovascularization
- Cancer Research
  - endothelial cell marker for neovascularization
- Genetics Research
  - Tissue/Cell Markers: endothelial cell markers for neovascularization
  - Tissue/Cell Markers

Mammalian Phenotype Terms by Genotype

References

Additional - Rag1^tm1Mom related

Additional - Tg(TIE2GFP)287Sato related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Laragen
- Standard PCR:Rag1Alternate1
- QPCR:humanized GFP/ER fusion qPCR
- QPCR:humanized GFP/ER fusion qPCR
- Probe:Rag1 Probe
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygous for the Rag1^tm1Mom allele and homozygous for the Tg(TIE2GFP)287Sato transgene.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Rag1^tm1Mom Tg(TIE2GFP)287Sato/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006770 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Rag1<tm1Mom>, Hemizygous for Tg(TIE2GFP)287Sato

Related Products and Services

Frozen Mouse Embryo	STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J	$2595.00
Frozen Mouse Embryo	STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J	$2595.00
Frozen Mouse Embryo	STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J	$3373.50
Frozen Mouse Embryo	STOCK Rag1<tm1Mom> Tg(TIE2GFP)287Sato/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Rag1tm1Mom

Allele Symbol: Rag1tm1Mom

Tg(TIE2GFP)287Sato

Allele Symbol: Tg(TIE2GFP)287Sato

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1tm1Mom related

Mammalian Phenotype Terms by Genotype

References

Additional - Rag1tm1Mom related

Additional - Tg(TIE2GFP)287Sato related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Genotype: Rag1^tm1Mom related

Additional - Rag1^tm1Mom related