B6;129P2-Rph3a^tm1Sud/J

Stock number: 006374
Product name: rabphilin KO
Research areas: Neurobiology Research

Description

No abnormalities were observed in these Rph3a knock-out mice, and they may be useful studies of synaptic vesicle exocytosis in neurons.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No protein product from the targeted gene is detected in brain tissue. Systematic quantitative analysis of the levels of 40 proteins known to interact with Rab3’s detected no significant changes as compared to normal brain composition. Immunocytochemical analysis indicates that the overall structure of the brain appears normal. No abnormalities in synaptic transmission or plasticity are observed. This mutant mouse strain represents a model that may be useful in studies of synaptic vesicle exocytosis in neurons.

Development

A targeting vector containing a neomycin resistance gene was used to replace two exons encoding residues 74-146 of the gene. The construct was electroporated into 129P2/OlaHsd–derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were crossed with C57BL/6 female mice. The strain was backcrossed to C57BL/6 for several generations by the donating laboratory.

Allele

Symbol: Rph3a^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:2179730
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rph3a
Marker name: rabphilin 3A
Chromosome: 5
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance cassette replaced two exons encoding residues 74-146 of the encoded protein. Western blot analysis on brain tissues of homozygous mice confirmed that no encoded protein was detected.