No abnormalities were observed in these Rph3a knock-out mice, and they may be useful studies of synaptic vesicle exocytosis in neurons.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No protein product from the targeted gene is detected in brain tissue. Systematic quantitative analysis of the levels of 40 proteins known to interact with Rab3’s detected no significant changes as compared to normal brain composition. Immunocytochemical analysis indicates that the overall structure of the brain appears normal. No abnormalities in synaptic transmission or plasticity are observed. This mutant mouse strain represents a model that may be useful in studies of synaptic vesicle exocytosis in neurons.
A targeting vector containing a neomycin resistance gene was used to replace two exons encoding residues 74-146 of the gene. The construct was electroporated into 129P2/OlaHsd–derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were crossed with C57BL/6 female mice. The strain was backcrossed to C57BL/6 for several generations by the donating laboratory.
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||rabphilin KO|
|Gene Symbol and Name||Rph3a, rabphilin 3A|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A neomycin resistance cassette replaced two exons encoding residues 74-146 of the encoded protein. Western blot analysis on brain tissues of homozygous mice confirmed that no encoded protein was detected.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
Homozygotes are viable and fertile. When maintaining a live colony, homozygous mice may be bred together
When using the rabphilin KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006374 in your Materials and Methods section.