Overview

Also Known As:Srd5a3^fl

Srd5a3^fl mice have loxP sites flanking exons 2-4 of the steroid 5 alpha-reductase 3 gene. Srd5a3 encodes a polyprenol reductase required in N-linked glycosylation.

Donating Investigator

Cat Lutz

Genetic overview

Genetic Background	Generation
	F1 (2020-05-16 00:00:00)

Srd5a3^{tm1c(EUCOMM)Wtsi}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed))	Srd5a3	steroid 5 alpha-reductase 3

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Research Applications

Research Tools
Cell Biology Research

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Details

Detailed Description

Srd5a3^fl mice have loxP sites flanking exons 2-4 of the steroid 5 alpha-reductase 3 gene. Srd5a3 encodes a polyprenol reductase required in N-linked glycosylation. As phenotype data is generated it will be made available to the public.

Development

The L1L2_Bact_P cassette was inserted at a position on Chromosome 5 upstream of exon 2 of the steroid 5 alpha-reductase 3 ( Srd5a3) gene. The cassette is composed of a frt site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second frt site and a second loxP site. A third loxP site is inserted downstream of exon 4. The critical exons, 2-4, are thus flanked by loxP sites. The construct was introduced into C57BL/6N-derived JM8.N4 embryonic stem (ES) cells, and correctly targeted ES cells were injected into B6(Cg)-Tyr^c-2J/J (Stock No. 000058) blastocysts. The resulting chimeric males were bred to C57BL/6NJ (Stock No. 005304) females. Frozen sperm was imported to The Jackson Laboratory. To establish our live colony, an aliquot of frozen sperm was used to fertilize oocytes from B6N.ROSA26::FLPe mice (Stock No. 016226) to remove the frt-flanked neomycin and lacZ sequences from resulting offspring. Progeny were subsequently bred to C57BL/6NJ mice to remove the FLPe-expressing transgene.

Control Suggestions

Noncarrier
Wild-type from the colony
Heterozygote from the colony

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Genetics

Srd5a3^{tm1c(EUCOMM)Wtsi}

Allele Symbol: Srd5a3^{tm1c(EUCOMM)Wtsi}

Allele Name	targeted mutation 1c, Wellcome Trust Sanger Institute
Allele Type	Targeted (Conditional ready (e.g. floxed))
Allele Synonym(s)	Srd5a3^fl
Gene Symbol and Name	Srd5a3, steroid 5 alpha-reductase 3
Gene Synonym(s)
Strain of Origin	C57BL/6N-A^tm1Brd
Chromosome	5
Molecular Note	The L1L2_Bact_P cassette was inserted at position 76147218 of Chromosome 5 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 76150215. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
Cell Biology Research
- Post-translational Processing

Mammalian Phenotype Terms by Genotype

References

Additional - Srd5a3^{tm1c(EUCOMM)Wtsi} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Laragen
- Probe:Generic Cre Probe
- QPCR:Generic FLPe qPCR
- Probe:Generic FLP
- QPCR:Generic Neo Quantitative PCR-QPCR- 1.2
- Standard PCR:Generic LacZ
- Standard PCR:Tg(ACTFLPe)
- Standard PCR:Tg(ACTFLPe)
- Standard PCR:Generic Cre Melt Curve Analysis
- Standard PCR:Generic Neo
- Standard PCR:Generic LacZ Melt Curve Analysis
- Probe:Generic Neo
- Probe:Generic LacZ Probe
- QPCR:Generic LacZ QPCR Alternate 1
- Standard PCR:GTCA
- Standard PCR:Srd5a3
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred to non-carrier (wildtype) mice from the colony, or to C57BL/6NJ inbred mice (Stock No. 005304). As of January 2020, the viability and fertility of homozygous mice has not been assessed. Homozygous mice are expected to be viable and fertile.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x Wild-type
- Wild-type x Heterozygote
Citation
When using the Srd5a3^fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #034731 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

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Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Srd5a3fl

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Srd5a3tm1c(EUCOMM)Wtsi

Allele Symbol: Srd5a3tm1c(EUCOMM)Wtsi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Srd5a3tm1c(EUCOMM)Wtsi related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:Srd5a3^fl

Srd5a3^{tm1c(EUCOMM)Wtsi}

Allele Symbol: Srd5a3^{tm1c(EUCOMM)Wtsi}

Additional - Srd5a3^{tm1c(EUCOMM)Wtsi} related