These Plp1 knock-out mice exhibit no sign of motor deficits, tremors or seizures, in contrast to most naturally occurring PLP mutants.
Dr. Klaus-Armin Nave, Max-Planck-Institute of Experimental Med
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Plp1 | proteolipid protein (myelin) 1 |
The gene for myelin proteolipid protein (Plp) lies on the X chromosome. Mice homozygous or hemizygous for the Plptm1Kan targeted mutation are viable and fertile. Although they lack expression of the PLP protein, they show no sign of motor deficits, tremors or seizures, in contrast to most naturally occurring PLP mutants. PLP is a major membrane component of CNS myelin, and many PLP mutants show defects in myelin sheath formation. However, Plptm1Kan mice do not exhibit this dysmyelination. Some ultrastructural differences are observed in the myelin of Plptm1Kan mutant mice when compared to controls, including a less distinct difference between the major dense line (MDL) and the intraperiod line (IPL), which corresponds to reduced physical stability of the myelin sheath and suggests a function of PLP in maintenance of myelin architecture. Plptm1Kan mutant mice show no evidence of myelin breakdown with age.
Allele Name | targeted mutation 1, Klaus-Armin Nave |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Plp-; PLP/DM20 null; PLP-null |
Gene Symbol and Name | Plp1, proteolipid protein (myelin) 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | X |
Molecular Note | A neomycin resistance cassette introduced by homologous recombination replaced the 3' part of exon 1,including the translation start codon and a 2.5 kb fragment of the first intron of Plp ("PLP/DM20 gene"). Western blot analysis showed absence of both PLP and DM20 proteolipids proteins in hemizygous mutant mice. |
Mutations Made By | Dr. Klaus-Armin Nave, Max-Planck-Institute of Experimental Med |
Plp is X-linked. When maintaining a live colony, homozygous females may be bred to hemizygous males.
When using the PLP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003255 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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X linked - Homozygous Females and Hemizygous males for Plp1<tm1Kan>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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