B6.129-Plin1^tm1Chan/J

Stock number: 003870
Product name: Plin-
Research areas: Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

These Plin knock-out mice exhibit less body fat are resistant to diet-induced obesity.

Detailed description

Homozygous null Plin mice are viable and fertile. At birth they are normal in size and do not display any gross physical or behavioral abnormalities. No transcripts are detected and Western-blot analysis of adipocyte and testes indicate no protein products are present. Although null mice consume more food than wildtype littermates, they have significantly less body fat (30-74%) and exhibit smaller white adipocytes (62%). With diminished fat stores, the mice are cold sensitive under fasting conditions. A greater muscle mass allows them to maintain a normal body weight. Hormone sensitive lipase activity is greatly increased in null mice resulting in elevated levels of basal lipolysis. Null mice are resistant to diet-induced obesity. The inheritance of the null alleles in Lepr^db/db mice reverses their obesity phenotype.

Development

A targeting vector containing a neomycin resistance, IRES-beta-galactosidase and herpes simplex virus thymidine kinase genes was used to disrupt exons 2 and intron 1. The construct was electroporated into R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were backcrossed to C57BL/6J animals.

Allele

Symbol: Plin1^tm1Chan
Name: targeted mutation 1, Lawrence Chan
MGI accession ID: MGI:2176248
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Plin1
Marker name: perilipin 1
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: An IRES-lacZ gene followed by a neomycin resistance cassette replaced a genomic fragment containing part of exon 2 and intron 2. Northern blot analysis demonstrated that the encoded transcript was not detectable in homozygous mice. Western blot anlaysis on extracts derived from adipocytes and testis of homozygous mice confirmed that no detectable protein is expressed from this allele.