These Grm5 knock-out mice exhibit reduction of long-term potentiation in the NMDA receptor-dependent pathways and impairment in the acquisition and use of spatial information.
IMR Colony, The Jackson Laboratory
Long-term potentiation (LTP) in Grm5tm1Rod (mGluR5) targeted mutant mice was significantly reduced in the NMDA receptor (NMDAR)-dependent pathways such as the CA1 region and dentate gyrus of the hippocampus, whereas LTP remained intact in the mossy fiber synapses on the CA3 region, an NMDAR-independent pathway. These mutant mice were also impaired in the acquisition and use of spatial information in both the Morris water maze and contextual information in the fear-conditioning test. Homozygotes are viable and fertile.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The mutant allele was made by replacing the first exon of the Grm5 (mGluR5) (G protein coupled receptor, family C, group 1, member E) locus with a neo marker. As of 2013, this strain has been backcrossed onto C57BL/6 at least six generations.
|Allele Name||targeted mutation 1, John C Roder|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Gprc1etm1Rod; mGluR5-|
|Gene Symbol and Name||Grm5, glutamate receptor, metabotropic 5|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was designed to delete a 0.4 kb fragment containing part of exon 1 and a part of intron 1, with a neomycin resistance cassette. Western blot failed to detect protein in mutants.|
|Mutations Made By|| |
Dr. John Roder, University of Toronto
Homozygotes are viable and fertile. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the mGluR5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003558 in your Materials and Methods section.