Under control of a mouse prion protein promoter, which drives transgene expression in the neurons of the central nervous system, these transgenic mice express an N-terminally truncated human Huntingtin cDNA. They develop behavioral abnormalities, including loss of coordination, tremors, hypokinesis and abnormal gait, before dying prematurely.
Dr. David R Borchelt, University of Florida
Gabriele Schilling, Johns Hopkins University School of Medicine
Christopher Ross, Johns Hopkins University
Genetic Background | Generation |
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N78
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Starting at:
$278.00 Domestic price for female 4-week |
308.27 Domestic price for breeder pair |
Mice expressing this transgene appear normal at birth through 1-2 months. Mice fail to gain weight, develop tremors, hypokinesis and lack coordination. They exhibit an abnormal gait and frequent hind limb clasping. Life expectancy is 5-6 months. Studies using huntingtin antibodies indicated numerous immunoreactive nuclear inclusions in multiple neuron populations. Neuritic damage is evident.
This transgenic line expresses an N-terminally truncated human huntingtin cDNA that encodes 82 glutamines and encompasses the first 171 amino acids. The altered huntingtin cDNA is under control of a mouse prion protein promoter. Expression is observed in neurons of the central nervous system.
Expressed Gene | HTT, huntingtin, human |
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Site of Expression |
Allele Name | transgene insertion 81, Gabriele Schilling |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | HD-N171-82Q; N171-82Q; PrP-htt-N171-82Q; Tg(HD82Gln)81Dbo; TGN(HD82Gln)81Dbo |
Gene Symbol and Name | Tg(HD82Gln)81Gschi, transgene insertion 81, Gabriele Schilling |
Gene Synonym(s) | |
Promoter | Prn, prion protein readthrough transcript, mouse, laboratory |
Expressed Gene | HTT, huntingtin, human |
Strain of Origin | C3H/B6 |
Chromosome | UN |
Molecular Note | This transgenic line expresses an N-terminally truncated human huntingtin cDNA that encodes 82 glutamines and encompasses the first 171 amino acids. The altered huntingtin cDNA is under control of a mouse prion protein promoter, which drives transgene expression in the neurons of the central nervous system |
Mutations Made By | Dr. David Borchelt, University of Florida |
This strain originated on a C3H/B6 background. The strain is maintained by crossing hemizygous males with wildtype B6C3F1/J females. The donating investigator reports that hemizygous females tend to make poor mothers. Expected coat color from breeding:Agouti and Black
When using the N171-82Q mice mouse strain in a publication, please cite the originating article(s) and include JAX stock #003627 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrierfor Tg(HD82Gln)81Dbo |
Frozen Mouse Embryo | B6C3-Tg(HD82Gln)81Gschi/J | $2595.00 |
Frozen Mouse Embryo | B6C3-Tg(HD82Gln)81Gschi/J | $2595.00 |
Frozen Mouse Embryo | B6C3-Tg(HD82Gln)81Gschi/J | $3373.50 |
Frozen Mouse Embryo | B6C3-Tg(HD82Gln)81Gschi/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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