C;129P2-Npy2r^tm1Pern/J

Stock number: 005417
Product name: Y2-
Research areas: Internal/Organ Research, Neurobiology Research

Description

These Npy2r knock-out mice exhibit increased night time basal heart rate , reduced activity levels, increased food intake, and delayed wound healing in the skin.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile and do not display any gross physical or behavioral abnormalities. By 1 month of age, mutant mice exhibit mild obesity. High-fat (Western) diet enhances the weight increase in female mutants. Northern blot analysis of hypothalamus detects mRNA encoding beta-galactosidase and not the endogenous mRNA. Beta galactosidase activity pattern mimic the endogenous gene expression pattern. Y2 receptor binding experiments confirm loss of activity. Mice homozygous for the mutation have increased night time basal heart rate compared to wildtype and exhibit reduced activity levels. Increased food intake is observed. Short term leptin administration elicits a decreased response of 15.2% food intake reduction compared to the 30.5% reduction seen in wildtype controls. Mutant mice are more sensitive to sodium Phenobarbital-induced sedation. Treatment with neuropeptide Y does not induce angiogenesis in the cornea. Wound healing in the skin is delayed with reduced neovascularization. This mutant mouse strain may be useful in studies of the role of the neuropeptide Y receptor and the physiological effects of neuropeptide Y.

Development

A targeting vector containing an internal ribosome entry site (IRES)-tau-lacZ fusion gene, and neomycin resistance gene and herpes simplex virus thymidine kinase genes was used to disrupt the protein encoding exon. The construct was electroporated into 129P2/OlaHsd derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then intercrossed to produce homozygotes.

Allele

Symbol: Npy2r^tm1Pern
Name: targeted mutation 1, Patrick Ernfors
MGI accession ID: MGI:2654580
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Npy2r
Marker name: neuropeptide Y receptor Y2
Chromosome: 3
Strain of origin: 129/Sv
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: The gene was disrupted by insertion of a lacZ-neo cassette into a protein-coding exon via homologous recombination. Absence of gene expression was confirmed by Northern blot analysis of hypothalamus RNA.