These Npy2r knock-out mice exhibit increased night time basal heart rate , reduced activity levels, increased food intake, and delayed wound healing in the skin.
Patrik Ernfors, Karolinska Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Npy2r | neuropeptide Y receptor Y2 |
Mice that are homozygous for the targeted mutation are viable, fertile and do not display any gross physical or behavioral abnormalities. By 1 month of age, mutant mice exhibit mild obesity. High-fat (Western) diet enhances the weight increase in female mutants. Northern blot analysis of hypothalamus detects mRNA encoding beta-galactosidase and not the endogenous mRNA. Beta galactosidase activity pattern mimic the endogenous gene expression pattern. Y2 receptor binding experiments confirm loss of activity. Mice homozygous for the mutation have increased night time basal heart rate compared to wildtype and exhibit reduced activity levels. Increased food intake is observed. Short term leptin administration elicits a decreased response of 15.2% food intake reduction compared to the 30.5% reduction seen in wildtype controls. Mutant mice are more sensitive to sodium Phenobarbital-induced sedation. Treatment with neuropeptide Y does not induce angiogenesis in the cornea. Wound healing in the skin is delayed with reduced neovascularization. This mutant mouse strain may be useful in studies of the role of the neuropeptide Y receptor and the physiological effects of neuropeptide Y.
A targeting vector containing an internal ribosome entry site (IRES)-tau-lacZ fusion gene, and neomycin resistance gene and herpes simplex virus thymidine kinase genes was used to disrupt the protein encoding exon. The construct was electroporated into 129P2/OlaHsd derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then intercrossed to produce homozygotes.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Patrick Ernfors |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Npy2r-; Y2- |
Gene Symbol and Name | Npy2r, neuropeptide Y receptor Y2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129/Sv |
Chromosome | 3 |
Molecular Note | The gene was disrupted by insertion of a lacZ-neo cassette into a protein-coding exon via homologous recombination. Absence of gene expression was confirmed by Northern blot analysis of hypothalamus RNA. |
Mutations Made By | Patrik Ernfors, Karolinska Institute |
When maintaining a live colony, these mice are bred as homozygotes. By 1 month of age, mutant mice exhibit mild obesity.
When using the Y2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005417 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Npy2r<tm1Pern> |
Frozen Mouse Embryo | C;129P2-Npy2r<tm1Pern>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C;129P2-Npy2r<tm1Pern>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C;129P2-Npy2r<tm1Pern>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C;129P2-Npy2r<tm1Pern>/J Frozen Embryos | $3373.50 |
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