IL-1RAcP-null (Il1rap knock-out) mice exhibit loss of IL-1 receptor mediated signal transduction. These mice may be useful in studying the role of IL-1 cytokine signaling in immune responses such as IL-33 receptor complexes and oncogenic signaling pathways (e.g., AML, CML).
Dr. Mark A. Labow, Novartis Corporation
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il1rap | interleukin 1 receptor accessory protein |
Il1rap encodes interleukin 1 receptor accessory protein (IL-1RAcP) which is a component of the interleukin-1 receptor complex, and is involved in interleukin-1 signalling. IL-1RAcP-null mice are viable and fertile. They exhibit loss of IL-1 receptor-mediated signal transduction. Treatment of these mutant mice with IL-1 Alpha or IL-1 Beta results in no detectable increase in IL-6 levels in serum and fails to induce increased expression of E-selectin mRNA. Homozygous mice are viable with normal breeding.
A targeting vector was designed to replace a genomic fragment containing exons D1 and part of D2A (the exons that encode the first Ig domain and part of the second Ig domain) of the interleukin 1 receptor accessory protein gene (Il1rap) on chromosome 16 with a neomycin resistance cassette. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Mice are reported to be on a mixed C57BL/6;129S1 genetic background.
Allele Name | targeted mutation 1, Mark A Labow |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-1RAcP- |
Gene Symbol and Name | Il1rap, interleukin 1 receptor accessory protein |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 16 |
Molecular Note | A neomycin resistance cassette replaced a genomic fragment containing exons D1 and part of D2A. These exons encode the first Ig domain and part of the second Ig domain. Northern blot analysis revealed that a truncated mRNA was produced from this allele, but equilibrium binding studies demonstrated that no protein was present on the cell surface of primary mouse embryo fibroblasts (PMEFs) derived from homozygous mice. |
Mutations Made By | Dr. Mark Labow, Novartis Corporation |
Homozygous mice are viable with normal breeding. When maintaining a live colony, homozygous mice may be bred together.
When using the IL-1R AcP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003284 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Il1rap<tm1Roml> |
Frozen Mouse Embryo | B6;129S1-Il1rap<tm1Roml>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Il1rap<tm1Roml>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S1-Il1rap<tm1Roml>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S1-Il1rap<tm1Roml>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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