Nrxn1flox mice possess loxP sites flanking exon 21 of the neurexin I (Nrxn1) gene. NRXN1 encodes a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. This gene has also been identified as a candidate gene for Schizophrenia, autism, and intellectual disability. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 21 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 21, and a frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site, downstream of exon 21 of the neurexin I (Nrxn1) gene. Also, the splice site 4 upstream of exon 21 was mutated to a canonical splice acceptor sequence. The construct was electroporated into C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Upon arrival, Nrxn1flox mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 5, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Nrxn1, neurexin I|
|Strain of Origin||C57BL/6|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 21, and a frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site, downstream of exon 21. Also, splice site 4, upstream of exon 21, was mutated to a canonical splice acceptor sequence.|
When maintaining a live colony, homozygous mice may be bred together.
When using the C57BL/6-Nrxn1tm5Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #026685 in your Materials and Methods section.