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This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Transgene = ~200 bp
Internal positive control = 324 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
19974 | GGC CAG GCT GTT CTT CTT AG | Transgene Reverse | A | ERT2 | ||
oIMR7338 | CTA GGC CAC AGA ATT GAA AGA TCT | Internal Positive Control Forward | A | |||
oIMR7339 | GTA GGT GGA AAT TCT AGC ATC ATC C | Internal Positive Control Reverse | A | |||
oIMR8346 | ATA CCG GAG ATC ATG CAA GC | Transgene Forward | A | Cre |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.30 X |
MgCl2 | 2.60 mM |
dNTP KAPA | 0.26 mM |
19974 | 0.50 uM |
oIMR7338 | 0.50 uM |
oIMR7339 | 0.50 uM |
oIMR8346 | 0.50 uM |
Glycerol | 6.50 % |
Dye | 1.00 X |
Kapa 2G HS taq polym | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles (Touchdown) | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
037511 | B6(129S4)-Pou2f3tm1.1(cre/ERT2)Imt/J |
038514 | B6(129S6)-Tg(Slc6a5-cre/ERT2)#Uze/J |
038595 | B6(Cg)-Dcxem1(cre/ERT2)Jjan/J |
026802 | B6(Cg)-Ins1tm2.1(cre/ERT2)Thor/J |
032758 | B6(Cg)-Tg(Acta2-cre/ERT2)1Ikal/J |
038495 | B6.129S6(Cg)-Gli1tm3(cre/ERT2)Alj/RtigJ |
038129 | B6.Cg-Ascl3tm2.1(EGFP/icre/ERT2)Ovi/J |
037804 | B6.Cg-Clec9atm4.1(cre/ERT2)Crs/J |
036450 | B6.Cg-Foxl1tm2(cre/ERT2)Khk/Mmjax |
035169 | B6.Cg-Krastm4Tyj Apctm1Tno Tg(CDX2-cre/ERT2)752Erf/MaraJ |
038956 | B6.Cg-Npr3tm2(cre/ERT2)Hze/J |
038834 | B6.Cg-Sdk1tm1.1(cre/ERT2)Jrs/KrishJ |
038840 | B6.Cg-Sdk2tm1.1(cre/ERT2)Jrs/KrishJ |
029567 | B6.Cg-Tg(Col1a2-cre/ERT,-ALPP)7Cpd/2J |
016237 | B6.Cg-Tg(Col1a2-cre/ERT,-ALPP)7Cpd/J |
008538 | B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J |
032757 | B6.Cg-Tg(Dmp1-cre/ERT2)0022Pdp/IkalJ |
012849 | B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J |
024709 | B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J |
038390 | B6.Cg-Tg(KRT14-cre/ERT2)1Ipc/AkinJ |
029684 | B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J |
005975 | B6.Cg-Tg(Plp1-cre/ERT)3Pop/J |
029211 | B6.Cg-Tg(Prrx1-cre/ERT2,-EGFP)1Smkm/J |
013590 | B6.Cg-Tg(Tyr-cre/ERT2)13Bos Braftm1Mmcm Ptentm1Hwu/BosJ |
020282 | B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J |
029231 | B6;129-Bhlha15tm4Skz/J |
036292 | B6;129-Gt(ROSA)26Sortm1(BEST1-cre/ERT2)Vold/J |
036300 | B6;129S4-Cux1tm1(cre/ERT2)Zjh/J |
036296 | B6;129S4-Fezf2tm1.1(cre/ERT2)Zjh/J |
030346 | B6;129S4-Gcgem1(cre/ERT2)Khk/Mmjax |
036298 | B6;129S4-Tle4tm1.1(cre/ERT2)Zjh/J |
012362 | B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J |
014160 | B6;DBA-Tg(S100b-EGFP/cre/ERT2)22Amc/J |
035554 | B6;FVB-Tg(Lfng-cre/ERT2)1Mmsa/J |
036751 | B6;SJL-Gfralem2(cre/ERT2)Rsy/J |
036770 | B6;SJL-Tg(Gm5127-cre/ERT2)1Mhsi/J |
037087 | B6J.Cg-Tg(Mnx1-cre/ERT2)8Rbro/Mmjax |
018280 | B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J |
033019 | B6N.Cg-Tg(Vil1-cre)20Syr/J |
031008 | B6N.FVB-Tg(Aldh1l1-cre/ERT2)1Khakh/J |
038602 | C57BL/6-Car4em1(cre/ERT2)Jich/J |
038831 | C57BL/6-Efnb2em1(cre/ERT2)Kloh/J |
038640 | C57BL/6-Rtkn2em1(cre/ERT2)Jich/J |
036499 | C57BL/6-Tg(Aire-cre/Esr1)1Mmat/J |
034244 | C57BL/6-Tg(Atp4b-cre/ERT2)1Jrgo/J |
037658 | C57BL/6-Tg(Myh11-cre/ERT2)F31Gko/J |
036217 | C57BL/6-Tg(Nanog-cre/Esr1*)4470Mms/Mmjax |
016261 | C57BL/6-Tg(Nes-cre/ERT2)KEisc/J |
037467 | C57BL/6-Tg(Tal1-cre/ERT)1Jrg/J |
037466 | C57BL/6-Tg(Tal1-cre/ERT)42-056Jrg/J |
039217 | C57BL/6J-Dpp4em1(cre/ERT2)Jkpns/J |
039218 | C57BL/6J-Slc47a1em1(cre/ERT2)Jkpns/J |
037591 | C57BL/6J-Tg(Dct-cre/ERT2)40ALru/J |
036482 | C57BL/6J-Tg(Gata1-cre/ERT2)L259Jeng/J |
037269 | C57BL/6J-Tg(Krt8-cre/ERT2,-GFP)1Xin/J |
036976 | FVB.129S6(Cg)-Krt5tm1.1(cre/ERT2)Blh/MqrJ |
035703 | FVB.B6-Igfbp2em1(cre/ERT2)Ntang/J |
024760 | FVB/N-Tg(Ddx4-cre/ERT2)1Dcas/J |
036735 | STOCK Adgra3tm1.1(HBEGF,-cre/ERT2)Pac/Mmjax |
036942 | STOCK Agertm2.1(cre/ERT2)Blh/2J |
036343 | STOCK Apoc4em1(cre/ERT2)Hzhu/J |
036246 | STOCK Cyp1a2em1(cre/ERT2)Hzhu/J |
038582 | STOCK Fbxo2tm1.1(cre/ERT2)Akg/J |
038587 | STOCK Foxi3em2(cre/ERT2)Akg/J |
036247 | STOCK Gls2em1(cre/ERT2)Hzhu/J |
036248 | STOCK Glulem1(cre/ERT2)Hzhu/J |
039021 | STOCK Gpr83tm1.1(cre/ERT2)Ddg/J |
024283 | STOCK Hcn4tm2.1(cre/ERT2)Sev/J |
032429 | STOCK Krastm4Tyj Trp53tm1Brn Tg(Pdx1-cre/Esr1*)#Dam/J |
038257 | STOCK Krt13em1(cre/ERT2)Raja/J |
036293 | STOCK Lhx2tm1.1(cre/ERT2)Zjh/J |
032285 | STOCK Slc34a1tm1(EGFP/cre/ERT2)Bhum/J |
039022 | STOCK Tacr1tm1.1(cre/ERT2)Ddg/J |
010911 | STOCK Wt1tm1(EGFP/cre)Wtp/J |
010912 | STOCK Wt1tm2(cre/ERT2)Wtp/J |
025736 | STOCK Tg(Cela1-cre/ERT)1Lgdn/J |
036851 | STOCK Tg(KLKB1-cre/ERT2)1Pcn/CmoJ |
005107 | STOCK Tg(KRT14-cre/ERT)20Efu/J |
028364 | STOCK Tg(Neurod1-cre)1Able/J |
012586 | STOCK Tg(Slc1a3-cre/ERT)1Nat/J |
018829 | STOCK Tg(Sox9-cre/ERT2)1Msan/J |
012708 | STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ |
82 strains use this protocol |