The PSA-Cre-ERT2 transgene was designed with the human kallikrein B, plasma 1 (KLKB1 or PSA) proximal promoter and androgen-dependent enhancer sequences driving expression of a cre/ERT2 fusion gene. PSA encodes a glycoprotein expressed in prostatic epithelium that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Restricted to the cytoplasm, cre/ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. Mice homozygous for the PSA-Cre-ERT2 transgene are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring. When bred with mice carrying a floxed sequence in the Pten gene (see Stock No. <a href="https://www.jax.org/strain/006440">006440</a>), resulting mice exhibit prostate epithelium hyperplasia within 4 weeks after PTEN ablation with tamoxifen and prostatic intraepithelial neoplasia (PIN) in all lobes within 2-3 months. Eight to 10 months after PTEN ablation, some PINs of the dorsolateral lobe progress to adenocarcinoma, similar to the course of human cancer formation.

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The PSA-Cre-ERT2 transgene was designed with the human KLKB1 (or PSA) proximal promoter and androgen-dependent enhancer sequences driving expression of a cre/ERT2 fusion gene. They are useful for applications requiring timed, cell type-specific knock-outs in the prostatic epithelium.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.

STOCK Tg(KLKB1-cre/ERT2)1Pcn/CmoJ - Embryo